P2Y1 Receptor Signaling Contributes to High Salt-Induced Priming of the NLRP3 Inflammasome in Retinal Pigment Epithelial Cells

• Published in: PloS one Vol. 11; no. 10; p. e0165653
• Main Authors: Prager, Philipp, Hollborn, Margrit, Steffen, Anja, Wiedemann, Peter, Kohen, Leon, Bringmann, Andreas
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.10.2016; Public Library of Science (PLoS)
• Subjects: 1-Phosphatidylinositol 3-kinase; Activation; Age; Autocrine signalling; Biology and Life Sciences; Caspase-1; Cell culture; Cells, Cultured; Cytokines; Diet; Dietary intake; Enzyme-linked immunosorbent assay; Ethics; Extracellular signal-regulated kinase; Gene Expression; Glucocorticoids; Human subjects; Humans; Hypertension; Hypoxia; Inflammasomes; Inflammasomes - metabolism; Inositol 1,4,5-trisphosphate receptors; Interleukin 18; JNK protein; Kinases; Lymphocytes T; Macular degeneration; MAP kinase; Medicine and Health Sciences; NLR Family, Pyrin Domain-Containing 3 Protein - genetics; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; Osmolarity; Osmotic pressure; Paracrine signalling; Phosphatase; Phospholipase; Phospholipase C; Physical Sciences; Polymerase chain reaction; Priming; Proteins; Receptor mechanisms; Receptors; Receptors, Purinergic P2Y1 - metabolism; Research and Analysis Methods; Retina; Retinal Pigment Epithelium - cytology; Retinal Pigment Epithelium - metabolism; Risk factors; Salt; Salts; Signal Transduction; Sodium chloride; Sodium Chloride - administration & dosage; Sucrose; Sugar; Transcription factors; Transcription Factors - metabolism; Vascular endothelial growth factor; United Kingdom > UK; Germany

Systemic hypertension is a risk factor of age-related macular degeneration (AMD), a chronic inflammatory disease. Acute hypertension is caused by increased extracellular osmolarity after intake of dietary salt (NaCl). We determined in cultured human retinal pigment epithelial (RPE) cells whether hig...