Partial gene suppression improves identification of cancer vulnerabilities when CRISPR-Cas9 knockout is pan-lethal

• Published in: Genome Biology Vol. 24; no. 1; pp. 1 - 192
• Main Authors: Krill-Burger, J. Michael, Dempster, Joshua M., Borah, Ashir A., Paolella, Brenton R., Root, David E., Golub, Todd R., Boehm, Jesse S., Hahn, William C., McFarland, James M., Vazquez, Francisca, Tsherniak, Aviad
• Format: Journal Article
• Language: English
• Published: London BioMed Central 23.08.2023; BMC
• Subjects: Cancer; Cancer genomics; Cell viability; CRISPR; CRISPR-Cas systems; CRISPR-Cas9 genome editing; Datasets; Experiments; Functional genomics; Genes; Genomes; Mutation; Reagents; RNA-mediated interference; RNAi; Target identification; Tumor cell lines

BackgroundHundreds of functional genomic screens have been performed across a diverse set of cancer contexts, as part of efforts such as the Cancer Dependency Map, to identify gene dependencies—genes whose loss of function reduces cell viability or fitness. Recently, large-scale screening efforts ha...