Reduced fire blight susceptibility in apple cultivars using a high‐efficiency CRISPR/Cas9‐FLP/FRT‐based gene editing system

• Published in: Plant biotechnology journal Vol. 18; no. 3; pp. 845 - 858
• Main Authors: Pompili, Valerio, Dalla Costa, Lorenza, Piazza, Stefano, Pindo, Massimo, Malnoy, Mickael
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.03.2020; John Wiley and Sons Inc
• Subjects: Agrobacterium radiobacter; Agrobacterium tumefaciens; Apple; Apples; bacteria; biotechnology; Blight; Cassettes; Cloning; Comparative analysis; CRISPR; CRISPR-Cas systems; Crop diseases; Cultivars; Deoxyribonucleic acid; DIPM; DNA; DNA sequencing; Efficiency; Erwinia amylovora; fire blight; FLP/FRT recombination; FRT gene; Fruits; gene editing; Gene expression; Genes; genetic markers; Genetic modification; Genetically altered foods; genetically modified organisms; Genomes; Health aspects; heat treatment; Kinases; loss-of-function mutation; Malus domestica; Malus × domestica; Mutation; Neomycin; Pathogens; Proteins; quantitative polymerase chain reaction; Recombination; Signal transduction; Malus × domestica; DIPM; fire blight; gene editing; FLP/FRT recombination

Summary The bacterium Erwinia amylovora, the causal agent of fire blight disease in apple, triggers its infection through the DspA/E effector which interacts with the apple susceptibility protein MdDIPM4. In this work, MdDIPM4 knockout has been produced in two Malus × domestica susceptible cultivars...