Reduced fire blight susceptibility in apple cultivars using a high‐efficiency CRISPR/Cas9‐FLP/FRT‐based gene editing system - Pompili, Valerio; Dalla Costa, Lorenza; Piazza, Stefano; Pindo, Massimo; Malnoy, Mickael (Plant biotechnology journal, 2020)
Keywords: Agrobacterium radiobacter; Agrobacterium tumefaciens; Apple; Apples; bacteria; biotechnology; Blight; Cassettes; Cloning; Comparative analysis; CRISPR; CRISPR-Cas systems; Crop diseases; Cultivars; Deoxyribonucleic acid; DIPM; DNA; DNA sequencing; Efficiency; Erwinia amylovora; fire blight; FLP/FRT recombination; FRT gene; Fruits; gene editing; Gene expression; Genes; genetic markers; Genetic modification; Genetically altered foods; genetically modified organisms; Genomes; Health aspects; heat treatment; Kinases; loss-of-function mutation; Malus domestica; Malus × domestica; Mutation; Neomycin; Pathogens; Proteins; quantitative polymerase chain reaction; Recombination; Signal transduction; Malus × domestica; DIPM; fire blight; gene editing; FLP/FRT recombination