Establishment of a panel of in-house polyclonal antibodies for the diagnosis of enterovirus infections

• Published in: Neuropathology Vol. 35; no. 2; pp. 107 - 121
• Main Authors: Kotani, Osamu, Iwata-Yoshikawa, Naoko, Suzuki, Tadaki, Sato, Yuko, Nakajima, Noriko, Koike, Satoshi, Iwasaki, Takuya, Sata, Tetsutaro, Yamashita, Teruo, Minagawa, Hiroko, Taguchi, Fumihiro, Hasegawa, Hideki, Shimizu, Hiroyuki, Nagata, Noriyo
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01.04.2015; Wiley Subscription Services, Inc; John Wiley and Sons Inc
• Subjects: Animals; Antibodies, Viral - immunology; Antigens; Capsid Proteins - immunology; Coxsackievirus B3; Coxsackievirus Infections - diagnosis; Coxsackievirus Infections - immunology; Echovirus Infections - diagnosis; Echovirus Infections - immunology; Enterovirus; Enterovirus - classification; Enterovirus - immunology; Enterovirus - isolation & purification; Enterovirus Infections - diagnosis; Enterovirus Infections - immunology; Evaluation Studies as Topic; Fluorescent Antibody Technique, Indirect; Humans; Immunohistochemistry; Infections; Medical research; Mice; Original; paraffin-embedded tissue; Picornaviridae; Poliovirus; Polyclonal antibodies; polyclonal antibody; Rodents; Sensitivity and Specificity; Serotyping; viral encephalitis; paraffin-embedded tissue; viral encephalitis; immunohistochemistry; enterovirus; polyclonal antibody

The aim of this study was to establish a reliable method of virus detection for the diagnosis of critical enterovirus infections such as acute infective encephalitis, encephalomyelitis and myocarditis. Because histopathological and immunohistochemical analyses of paraffin‐embedded tissues play an im...