Image Analysis of Eosinophil Peroxidase Immunohistochemistry for Diagnosis of Eosinophilic Esophagitis

• Published in: Digestive diseases and sciences Vol. 66; no. 3; pp. 775 - 783
• Main Authors: Wright, Benjamin L., Doyle, Alfred D., Shim, Kelly P., Pai, Rish K., Barshow, Suzanne M., Horsley-Silva, Jennifer L., Luo, Huijun, Rank, Matthew A., Jacobsen, Elizabeth A., Katzka, David A., Kita, Hirohito, Dellon, Evan S.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2021; Springer; Springer Nature B.V
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Analysis; Antibodies; Autoimmune diseases; Automation; Biochemistry; Biopsy; Case-Control Studies; Diagnosis; Digitization; Disease; Dysphagia; Endoscopy; Eosinophil Peroxidase - analysis; Eosinophilic Esophagitis - diagnosis; Eosinophils - enzymology; Equipment and supplies; Esophageal diseases; Esophagitis; Esophagus; Esophagus - pathology; Ethanol; Female; Gastroenterology; Gastroesophageal reflux; Gastrointestinal diseases; Hepatology; Humans; Image processing; Image Processing, Computer-Assisted - methods; Immunohistochemistry; Immunohistochemistry - methods; Inflammation; Male; Medical diagnosis; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original Article; Peroxidase; Prospective Studies; Questionnaires; Retrospective Studies; Staining and Labeling - methods; Stains & staining; Transplant Surgery; Young Adult; Degranulation; Eosinophil peroxidase; Image analysis; Biomarker; Eosinophilic esophagitis

Background Diagnosis of eosinophilic esophagitis (EoE) requires manual quantification of tissue eosinophils. Eosinophil peroxidase (EPX) is an eosinophil-specific, cytoplasmic granule protein released during degranulation. Aims The objective of this study was to evaluate image analysis of EPX immuno...