Joint profiling of histone modifications and transcriptome in single cells from mouse brain

• Published in: Nature methods Vol. 18; no. 3; pp. 283 - 292
• Main Authors: Zhu, Chenxu, Zhang, Yanxiao, Li, Yang Eric, Lucero, Jacinta, Behrens, M. Margarita, Ren, Bing
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2021; Nature Publishing Group
• Subjects: 631/208/177; 631/61/212; Animals; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Brain; Brain mapping; Cell Line, Tumor; Chromatin; Chromatin - metabolism; Cortex (frontal); Epigenesis, Genetic - genetics; Epigenetics; Frontal Lobe - cytology; Frontal Lobe - metabolism; Gene expression; Gene Expression Profiling; Gene Expression Regulation - genetics; Gene mapping; Gene regulation; Genetic aspects; Genetic regulation; Genetic research; Genomes; HeLa Cells; High-throughput screening (Biochemical assaying); Hippocampus; Hippocampus - cytology; Hippocampus - metabolism; Histone Code - genetics; Histones; Humans; Life Sciences; Male; Mice; Mice, Inbred C57BL; Post-translational modification; Protein Processing, Post-Translational; Proteomics; Regulatory mechanisms (biology); Regulatory sequences; Regulatory Sequences, Nucleic Acid - genetics; Single-Cell Analysis; Structure; Transcriptome - genetics; Transcriptomes

Genome-wide profiling of histone modifications can reveal not only the location and activity state of regulatory elements, but also the regulatory mechanisms involved in cell-type-specific gene expression during development and disease pathology. Conventional assays to profile histone modifications...