Enzyme-catalysed [4+2] cycloaddition is a key step in the biosynthesis of spinosyn A

• Published in: Nature (London) Vol. 473; no. 7345; pp. 109 - 112
• Main Authors: Kim, Hak Joong, Ruszczycky, Mark W., Choi, Sei-hyun, Liu, Yung-nan, Liu, Hung-wen
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.05.2011; Nature Publishing Group
• Subjects: 631/1647/666/2259; 639/638/403/934; 639/638/92/60; Analytical, structural and metabolic biochemistry; Bacterial Proteins - biosynthesis; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; Biological and medical sciences; Biosynthesis; Catalysis; Chemical properties; Chromatography, High Pressure Liquid; Composition; E coli; Enzymes; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Humanities and Social Sciences; Insecticides; Investigations; Kinetics; letter; Macrolides - chemistry; Macrolides - metabolism; Mass spectrometry; Metabolites; Miscellaneous; multidisciplinary; Proteins; Saccharopolyspora - enzymology; Science; Science (multidisciplinary); Secondary metabolites; Spectrum analysis; United States; Cycloaddition; Insecticide; Enzyme; Pesticides; Prokaryote; Bacteria; Biosynthesis; Microorganism

A 'Diels–Alderase' enzyme identified The naturally occurring compound spinosyn A is a component of several environmentally benign commercial insecticides. How its tetracyclic ring system is biosynthesized has been a subject of much speculation. One possible mechanism is the Diels–Alder rea...