Determination of Polycyclic Aromatic Hydrocarbons in Water by Solid-Phase Microextraction and Liquid Chromatography

• Published in: Analytical Sciences Vol. 20; no. 10; pp. 1383 - 1388
• Main Author: CHEN, Hong-Wen
• Format: Journal Article
• Language: English
• Published: Japan The Japan Society for Analytical Chemistry 2004; Japan Science and Technology Agency
• Subjects: Chemistry Techniques, Analytical - methods; Chromatography, High Pressure Liquid - methods; Fluorescence; Hydrogen-Ion Concentration; Polycyclic Aromatic Hydrocarbons - analysis; Polycyclic Aromatic Hydrocarbons - chemistry; Temperature; Time Factors; Water - chemistry
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.20.1383

This study describes the determination of polycyclic aromatic hydrocarbons (PAHs) in water using high-performance liquid chromatography (HPLC) coupled with fluorescence detection (FLD). Because individual PAHs are generally present in water only at trace levels, a sensitive and accurate determination technique is essential. The separation and detection of five PAHs were run completely within 25 min by the HPLC/FLD system with an analytical C18 column, a fluorescence detection, and acetonitrile-water gradient elution. Calibration graphs were linear with very good correlation coefficients (r > 0.9998), and the detection limits were in the range of 2 - 6 ng/l for five PAHs. Solid phase microextraction (SPME) was performed for sample pretreatment prior to HPLC-FLD determination, and the governing parameters were investigated. Compared to conventional methods, SPME has high recovery, saves considerable time, and reduces solvents waste. The extraction efficiencies of five PAHs were above 88% and the extraction times were 35 min in one pretreatment procedure. One particular discovery is that 1.5 M sodium monochloroactate (ClCH2COONa) can improve the extraction yield of PAH compounds more than other inorganic salts. The SPME-HPLC-FLD technique provides a relatively simple, convenient, practical procedure, which was here successfully applied to determine five PAHs in water from authentic water samples.