Genotyping assay for differentiation of wild-type and vaccine viruses in subjects immunized with live attenuated influenza vaccine

• Published in: PloS one Vol. 12; no. 7; p. e0180497
• Main Authors: Matyushenko, Victoria, Isakova-Sivak, Irina, Smolonogina, Tatiana, Dubrovina, Irina, Tretiak, Tatiana, Rudenko, Larisa
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.07.2017; Public Library of Science (PLoS)
• Subjects: Age; Assaying; Biology and life sciences; Children; Circulation; Clinical trials; Development and progression; Differentiation; Fragments; Gene sequencing; Genes; Genetic aspects; Genomes; Genotype; Genotyping; Humans; Immunization; Influenza; Influenza vaccines; Influenza Vaccines - genetics; Influenza Vaccines - therapeutic use; Influenza viruses; Influenza, Human - genetics; Influenza, Human - prevention & control; Influenza, Human - virology; Laboratory animals; Medical research; Medicine; Medicine and health sciences; Nasopharynx; Nasopharynx - virology; Orthomyxoviridae - genetics; Orthomyxoviridae - pathogenicity; Pandemics; Pharynx; Physiological aspects; Polymerase chain reaction; Prevention; Primers; Research and Analysis Methods; Russia; Studies; Vaccines; Vaccines, Attenuated - genetics; Vaccines, Attenuated - therapeutic use; Virology; Virus Replication - genetics; Viruses; Russia; Ann Arbor Michigan; United States > US; Bangladesh

Live attenuated influenza vaccines (LAIVs) are considered as safe and effective tool to control influenza in different age groups, especially in young children. An important part of the LAIV safety evaluation is the detection of vaccine virus replication in the nasopharynx of the vaccinees, with spe...