丙型肝炎患者血清蛋白质组差异表达的初步研究

目的观察丙型肝炎病毒（HCV）患者血清蛋白质组的变化情况，为研究丙型肝炎的发病机制及治疗提供新的线索。方法收集丙型肝炎患者和健康对照者血清，去除血清中高丰度蛋白；应用双向凝胶电泳技术构建2组研究对象血清二维电泳图谱，Image Master软件分析所得图谱，寻找差异蛋白质点；基质辅助激光解吸电离一飞行时间质谱法对2组间比较所得目标蛋白质点进行质谱鉴定。结果获得了HCV患者血清蛋白质双向电泳图谱，对表达差异2倍以上进行质谱鉴定，共鉴定出15种蛋白质。结论成功鉴定了15个HCV相关蛋白质，为进一步研究其发病机制及治疗手段提供了实验基础。...

Full description

Saved in:
Bibliographic Details
Published in西安交通大学学报（医学版） Vol. 34; no. 2; pp. 210 - 213
Main Author 代晓霞 王晓琴 寻萌 雷金娥 王聪 楚雍烈
Format Journal Article
LanguageChinese
Published 西安交通大学医学院公共卫生系,陕西西安,710061%西安交通大学医学院第一附属医院检验科,陕西西安,710061%免疫学与病源生物学系,陕西西安,710061 2013
Subjects
丙型肝炎
双向电泳
蛋白质组学
质谱
蛋白质组学
质谱
双向电泳
丙型肝炎
Online AccessGet full text
ISSN1671-8259

Cover

More Information
Summary:目的观察丙型肝炎病毒（HCV）患者血清蛋白质组的变化情况，为研究丙型肝炎的发病机制及治疗提供新的线索。方法收集丙型肝炎患者和健康对照者血清，去除血清中高丰度蛋白；应用双向凝胶电泳技术构建2组研究对象血清二维电泳图谱，Image Master软件分析所得图谱，寻找差异蛋白质点；基质辅助激光解吸电离一飞行时间质谱法对2组间比较所得目标蛋白质点进行质谱鉴定。结果获得了HCV患者血清蛋白质双向电泳图谱，对表达差异2倍以上进行质谱鉴定，共鉴定出15种蛋白质。结论成功鉴定了15个HCV相关蛋白质，为进一步研究其发病机制及治疗手段提供了实验基础。
Bibliography:hepatitis C; proteomics; two-dimensional electrophoresis; mass spectrum
DAI Xiao-xia1 , WANG Xiao-qin3 , XUN Meng2 , LEI Jin-e3 , WANG Cong3 , CHU Yong-lie2 （1. Department of Public Health; 2. Department of Immunology and Pathogenic Biology; 3. Clinical Laboratory of the First Affiliated Hospital, Medical School of Xi＇an Jiaotong University, Xi＇an 710061, China）
A Objective To study changes in disease-associated serum proteins in patients infected with hepatitis C virus （HCV） and provide novel clues to identify the molecular mechanism and therapy of HCV infection. Methods The serum proteins from HCV patients and health controls were profiled quantitatively, and two-dimen- sional electrophoresis was applied to separate and compare with pre-treated serum samples. The pretreatment step removed most of albumin from the serum. The gels were analyzed by ImageMaster software. The different proteins were identified by MALDI-TOF-MS. Results Comparing normal and HCV-infected serum samples and testing the proteins with mo
ISSN:1671-8259