A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

• Published in: Nature communications Vol. 10; no. 1; pp. 3963 - 11
• Main Authors: Young, Joanna, Dominicus, Caia, Wagener, Jeanette, Butterworth, Simon, Ye, Xingda, Kelly, Gavin, Ordan, Merav, Saunders, Becky, Instrell, Rachael, Howell, Michael, Stewart, Aengus, Treeck, Moritz
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.09.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 42/41; 45/41; 49/47; 631/326/417/2546; 631/326/417/2547; 631/326/417/2550; Animals; Cell Line; Cloning; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR; CRISPR-Cas Systems; Gene Knockout Techniques - methods; Gene Library; Genetic screening; Genome, Protozoan; Genomes; gRNA; Humanities and Social Sciences; Humans; In vivo methods and tests; Libraries; Mice, Inbred C57BL; multidisciplinary; Oligonucleotides; Parasites; Pools; Protozoa; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems; Science; Science (multidisciplinary); Toxoplasma - genetics; Toxoplasma - pathogenicity; Toxoplasmosis - genetics; Toxoplasmosis - parasitology; Toxoplasmosis - pathology; Virulence; Virulence factors; Virulence Factors - genetics

Genome-wide CRISPR screening is a powerful tool to identify genes required under selective conditions. However, the inherent scale of genome-wide libraries can limit their application in experimental settings where cell numbers are restricted, such as in vivo infections or single cell analysis. The...