A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice - Young, Joanna; Dominicus, Caia; Wagener, Jeanette; Butterworth, Simon; Ye, Xingda; Kelly, Gavin; Ordan, Merav; Saunders, Becky; Instrell, Rachael; Howell, Michael; Stewart, Aengus; Treeck, Moritz (Nature communications, 2019)
Keywords: 42/41; 45/41; 49/47; 631/326/417/2546; 631/326/417/2547; 631/326/417/2550; Animals; Cell Line; Cloning; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR; CRISPR-Cas Systems; Gene Knockout Techniques - methods; Gene Library; Genetic screening; Genome, Protozoan; Genomes; gRNA; Humanities and Social Sciences; Humans; In vivo methods and tests; Libraries; Mice, Inbred C57BL; multidisciplinary; Oligonucleotides; Parasites; Pools; Protozoa; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems; Science; Science (multidisciplinary); Toxoplasma - genetics; Toxoplasma - pathogenicity; Toxoplasmosis - genetics; Toxoplasmosis - parasitology; Toxoplasmosis - pathology; Virulence; Virulence factors; Virulence Factors - genetics