A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

• Published in: Scientific reports Vol. 7; no. 1; pp. 3645 - 12
• Main Authors: Tajika, Yuki, Murakami, Tohru, Iijima, Keiya, Gotoh, Hiroki, Takahashi-Ikezawa, Maiko, Ueno, Hitoshi, Yoshimoto, Yuhei, Yorifuji, Hiroshi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.06.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 101/47; 13/51; 13/56; 14/63; 631/1647/2163; 631/1647/245/2221; 631/1647/245/2225; 631/1647/328/2240; 631/1647/334; 64/60; Animals; Brain stem; Brain Stem - metabolism; Data Analysis; Data processing; Embryo, Mammalian - cytology; Embryo, Mammalian - metabolism; Embryos; Fluorescent Antibody Technique; Histocytochemistry; Humanities and Social Sciences; Humans; Image Processing, Computer-Assisted; Imaging, Three-Dimensional - instrumentation; Imaging, Three-Dimensional - methods; Larvae; Light microscopy; Mice; Microscopic analysis; Microscopy - methods; Microscopy - standards; Molecular Imaging - instrumentation; Molecular Imaging - methods; multidisciplinary; Options trading; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-03900-9

We have developed an imaging method designated as co rrelative light m icroscopy and b lock-face i maging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen se...