Potassium channel-based optogenetic silencing

• Published in: Nature communications Vol. 9; no. 1; pp. 4611 - 13
• Main Authors: Bernal Sierra, Yinth Andrea, Rost, Benjamin R., Pofahl, Martin, Fernandes, António Miguel, Kopton, Ramona A., Moser, Sylvain, Holtkamp, Dominik, Masala, Nicola, Beed, Prateep, Tukker, John J., Oldani, Silvia, Bönigk, Wolfgang, Kohl, Peter, Baier, Herwig, Schneider-Warme, Franziska, Hegemann, Peter, Beck, Heinz, Seifert, Reinhard, Schmitz, Dietmar
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.11.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 14/69; 42; 631/1647/2253; 631/378; 64/116; 64/60; 692/4019; 9/74; 96/95; Adenylyl Cyclases - genetics; Adenylyl Cyclases - metabolism; Adenylyl Cyclases - radiation effects; Animals; Animals, Genetically Modified; Biological activity; Cardiomyocytes; Channelrhodopsins - radiation effects; Gene Expression - genetics; Gene Expression - radiation effects; Genetics; HEK293 Cells; Humanities and Social Sciences; Humans; Hyperpolarization; Information processing; Light; Luminous intensity; Mice; Models, Animal; multidisciplinary; Myocytes, Cardiac - metabolism; Neurons - metabolism; Neurons - radiation effects; Optics; Optogenetics - methods; Potassium; Potassium Channels - genetics; Potassium Channels - metabolism; Potassium Channels - radiation effects; Regulatory sequences; Rhodopsin - pharmacology; Science; Science (multidisciplinary); Silencer Elements, Transcriptional; Temporal resolution; Zebrafish

Optogenetics enables manipulation of biological processes with light at high spatio-temporal resolution to control the behavior of cells, networks, or even whole animals. In contrast to the performance of excitatory rhodopsins, the effectiveness of inhibitory optogenetic tools is still insufficient....