A novel fluorescent probe-based flow cytometric assay for mineral-containing nanoparticles in serum

• Published in: Scientific reports Vol. 7; no. 1; pp. 5686 - 17
• Main Authors: Smith, Edward R., Hewitson, Tim D., Cai, Michael M. X., Aghagolzadeh, Parisa, Bachtler, Matthias, Pasch, Andreas, Holt, Stephen G.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.07.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 13/31; 14/34; 692/4022/1585/1732; 692/699/1585/104/1586; Animal models; Bone turnover; Flow cytometry; Humanities and Social Sciences; Immunomodulation; Kidney diseases; Kidney transplantation; Kidneys; multidisciplinary; Nanoparticles; Quantitation; Renal function; Ripening; Science; Science (multidisciplinary); Toxicity

Calciprotein particles, nanoscale aggregates of insoluble mineral and binding proteins, have emerged as potential mediators of phosphate toxicity in patients with Chronic Kidney Disease. Although existing immunochemical methods for their detection have provided compelling data, these approaches are...