Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA

• Published in: Scientific reports Vol. 10; no. 1; pp. 21442 - 12
• Main Authors: Brogna, Raffaele, Oldenhof, Harriëtte, Sieme, Harald, Wolkers, Willem F.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.12.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/2196/2197; 631/1647/527/2257; 631/45/147; 631/61/32; 692/53/2421; Adult; Biomarkers; Deoxyribonucleic acid; DNA; DNA Fingerprinting - methods; Drying; Feasibility Studies; Female; Filters; Fingerprinting; Fourier analysis; Fourier transforms; Humanities and Social Sciences; Humans; Hygroscopic Agents - chemistry; Infrared spectroscopy; Male; Middle Aged; multidisciplinary; Polyvinyls - chemistry; Principal Component Analysis; Principal components analysis; Protein Conformation, beta-Strand; Protein Stability; Proteins - chemistry; Relative humidity; Saliva; Saliva - chemistry; Science; Science (multidisciplinary); Shelf life; Specimen Handling - methods; Spectroscopy, Fourier Transform Infrared; Storage conditions; Sucrose; Young Adult

Saliva has been widely recognized as a non-invasive, painless and easy-to-collect bodily fluid, which contains biomarkers that can be used for diagnosis of both oral and systemic diseases. Under ambient conditions, salivary biomarkers are subject to degradation. Therefore, in order to minimize degra...