The coilin N-terminus mediates multivalent interactions between coilin and Nopp140 to form and maintain Cajal bodies

• Published in: Nature communications Vol. 13; no. 1; pp. 6005 - 13
• Main Authors: Courchaine, Edward, Gelles-Watnick, Sara, Machyna, Martin, Straube, Korinna, Sauyet, Sarah, Enright, Jade, Neugebauer, Karla M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.10.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/44; 13/51; 13/89; 14; 14/1; 14/19; 14/33; 14/35; 14/63; 38/109; 631/337/1645/1792; 631/45/612/1245; 631/80/642/1363; 9/10; Amino acids; Amino Acids - metabolism; Cajal bodies; Cell Nucleus - metabolism; Coiled Bodies - metabolism; Cytoplasm; Depletion; Evolution; Fibrils; HeLa Cells; Humanities and Social Sciences; Humans; Molecular interactions; multidisciplinary; N-Terminus; Nopp140 protein; Organelles; Proteins; Ribonucleoproteins, Small Nuclear - metabolism; RNA, Small Nuclear - metabolism; Scaffolding; Science; Science (multidisciplinary); snRNA; Splicing; Structure-function relationships

Cajal bodies (CBs) are ubiquitous nuclear membraneless organelles (MLOs) that concentrate and promote efficient biogenesis of snRNA-protein complexes involved in splicing (snRNPs). Depletion of the CB scaffolding protein coilin disperses snRNPs, making CBs a model system for studying the structure a...