Fusion protein strategies for cryo-EM study of G protein-coupled receptors

• Published in: Nature communications Vol. 13; no. 1; pp. 4366 - 11
• Main Authors: Zhang, Kaihua, Wu, Hao, Hoppe, Nicholas, Manglik, Aashish, Cheng, Yifan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.07.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 101/28; 631/45/612/194; 631/535/1258/1259; Adenosine; Cryoelectron Microscopy - methods; Crystallization; Crystallography; Electron microscopy; Fusion protein; G protein-coupled receptors; GTP-Binding Proteins - metabolism; Helices; Humanities and Social Sciences; Integral membrane proteins; Interrogation; multidisciplinary; Protein Structure, Secondary; Proteins; Receptors; Receptors, G-Protein-Coupled - metabolism; Science; Science (multidisciplinary); Signaling; Transducers

Single particle cryogenic-electron microscopy (cryo-EM) is used extensively to determine structures of activated G protein-coupled receptors (GPCRs) in complex with G proteins or arrestins. However, applying it to GPCRs without signaling proteins remains challenging because most receptors lack struc...