Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody

• Published in: Nature communications Vol. 9; no. 1; pp. 436 - 11
• Main Authors: Füzik, Tibor, Formanová, Petra, Růžek, Daniel, Yoshii, Kentaro, Niedrig, Matthias, Plevka, Pavel
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.01.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 101/1; 101/28; 13/106; 631/326/596/2148; 631/326/596/2557; 631/535/1258/1259; Antibodies, Neutralizing - biosynthesis; Antibodies, Neutralizing - chemistry; Antibodies, Viral - biosynthesis; Antibodies, Viral - chemistry; Cell Line, Tumor; Cryoelectron Microscopy; Detachment; Encephalitis; Encephalitis Viruses, Tick-Borne - genetics; Encephalitis Viruses, Tick-Borne - metabolism; Encephalitis Viruses, Tick-Borne - ultrastructure; Fab; Fragmentation; Fragments; Gene Expression; Genomes; Glycoproteins; Histidine; Humanities and Social Sciences; Humans; Hydrogen-Ion Concentration; Immunoglobulin Fab Fragments - biosynthesis; Immunoglobulin Fab Fragments - chemistry; Membrane fusion; Membrane Fusion - genetics; Membrane proteins; Membranes; Meningitis; Monoclonal antibodies; multidisciplinary; Neurons - pathology; Neurons - virology; Neutralization; Neutralizing; pH effects; Protein Domains; Protein Multimerization; Proteins; Science; Science (multidisciplinary); Tick-borne encephalitis; Viral envelope proteins; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - metabolism; Virion - genetics; Virion - metabolism; Virion - ultrastructure; Virions; Virus Internalization; Viruses

Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. Here, we present cryo-EM structures of the native TBEV virion and its complex with Fab fragments of neut...