Freeze-frame imaging of synaptic activity using SynTagMA

• Published in: Nature communications Vol. 11; no. 1; p. 2464
• Main Authors: Perez-Alvarez, Alberto, Fearey, Brenna C., O’Toole, Ryan J., Yang, Wei, Arganda-Carreras, Ignacio, Lamothe-Molina, Paul J., Moeyaert, Benjamien, Mohr, Manuel A., Panzera, Lauren C., Schulze, Christian, Schreiter, Eric R., Wiegert, J. Simon, Gee, Christine E., Hoppa, Michael B., Oertner, Thomas G.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.05.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 14; 14/10; 14/35; 14/69; 631/378/87; 631/443/376; 64/60; 64/86; Action Potentials; Animals; Axon guidance; Axons; Axons - metabolism; Biomarkers - metabolism; Brain; Brain research; Brain slice preparation; Calcium; Calcium imaging; Cell culture; Cells, Cultured; Female; Fluorescence; Gene mapping; Genetic code; Hippocampus - cytology; Humanities and Social Sciences; Imaging, Three-Dimensional; Light; Male; Mapping; Mice, Inbred C57BL; Microscopy; multidisciplinary; Neuroimaging; Neurons; Neurons - metabolism; Presynaptic Terminals - metabolism; Rats, Sprague-Dawley; Rats, Wistar; Science; Science (multidisciplinary); Synapses; Synapses - physiology; Synaptophysin - metabolism; Time Factors; Windows (intervals)

Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge. Here we introduce SynTagMA to tag active synapses in a...