Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA

• Published in: Nature communications Vol. 12; no. 1; pp. 5753 - 12
• Main Authors: Natarajan, Aravind, Han, Alvin, Zlitni, Soumaya, Brooks, Erin F., Vance, Summer E., Wolfe, Marlene, Singh, Upinder, Jagannathan, Prasanna, Pinsky, Benjamin A., Boehm, Alexandria, Bhatt, Ami S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 45; 45/77; 45/88; 631/1647/2017/2003; 631/326/421; 692/308/575; Coronavirus Nucleocapsid Proteins - genetics; Coronaviruses; COVID-19; COVID-19 - diagnosis; COVID-19 Nucleic Acid Testing - standards; Deoxyribonucleic acid; DNA; Epidemiology; Feces; Feces - virology; Humanities and Social Sciences; Humans; multidisciplinary; Phosphoproteins - genetics; Preservation; Preservation, Biological - standards; Preservatives; Reagent Kits, Diagnostic; Reference Standards; Ribonucleic acid; RNA; RNA, Viral - analysis; RNA, Viral - genetics; SARS-CoV-2 - genetics; SARS-CoV-2 - isolation & purification; Science; Science (multidisciplinary); Severe acute respiratory syndrome coronavirus 2; Specimen Handling - standards; Viral diseases; Viral Load - standards

Patients with COVID-19 shed SARS-CoV-2 RNA in stool, sometimes well after their respiratory infection has cleared. This may be significant for patient health, epidemiology, and diagnosis. However, methods to preserve stool, and to extract and quantify viral RNA are not standardized. We test the perf...