The molecular basis for SARS-CoV-2 binding to dog ACE2

• Published in: Nature communications Vol. 12; no. 1; pp. 4195 - 10
• Main Authors: Zhang, Zengyuan, Zhang, Yanfang, Liu, Kefang, Li, Yan, Lu, Qiong, Wang, Qingling, Zhang, Yuqin, Wang, Liang, Liao, Hanyi, Zheng, Anqi, Ma, Sufang, Fan, Zheng, Li, Huifang, Huang, Weijin, Bi, Yuhai, Zhao, Xin, Wang, Qihui, Gao, George F., Xiao, Haixia, Tong, Zhou, Qi, Jianxun, Sun, Yeping
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 07.07.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13/31; 631/326/596/4130; 692/699/255/2514; 82/103; 82/80; 82/83; ACE2; Affinity; Angiotensin; Angiotensin-converting enzyme 2; Angiotensin-Converting Enzyme 2 - chemistry; Angiotensin-Converting Enzyme 2 - metabolism; Animals; Atomic properties; Binding; Binding Sites; Cell Line; Conversion; COVID-19; Cricetinae; Crystal structure; Crystallography, X-Ray; Dogs; Domestic animals; Enzymes; HeLa Cells; Host range; Humanities and Social Sciences; Humans; Hydrogen bonding; Hydrogen bonds; Infectivity; multidisciplinary; Mutation; Pandemics; Peptidyl-dipeptidase A; Protein Binding; Protein Domains; Proteins; Receptors; SARS-CoV-2 - genetics; SARS-CoV-2 - metabolism; SARS-CoV-2 - physiology; Science; Science (multidisciplinary); Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - metabolism; Spike protein; Viral diseases; Virus Internalization

SARS-CoV-2 can infect many domestic animals, including dogs. Herein, we show that dog angiotensin-converting enzyme 2 (dACE2) can bind to the SARS-CoV-2 spike (S) protein receptor binding domain (RBD), and that both pseudotyped and authentic SARS-CoV-2 can infect dACE2-expressing cells. We solved th...