SARS-CoV-2 proteome microarray for global profiling of COVID-19 specific IgG and IgM responses

• Published in: Nature communications Vol. 11; no. 1; pp. 3581 - 11
• Main Authors: Jiang, He-wei, Li, Yang, Zhang, Hai-nan, Wang, Wei, Yang, Xiao, Qi, Huan, Li, Hua, Men, Dong, Zhou, Jie, Tao, Sheng-ce
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.07.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 49/79; 631/1647/2017/2065; 631/250/255/2514; 631/45/612/1256; Adult; Antibodies; Antibodies, Viral - immunology; Betacoronavirus - immunology; Coronavirus 3C Proteases; Coronavirus Infections - immunology; Coronavirus Nucleocapsid Proteins; Coronaviruses; COVID-19; Diagnostic systems; Enzyme-Linked Immunosorbent Assay; Female; Humanities and Social Sciences; Humans; Immune response; Immune system; Immunoglobulin G; Immunoglobulin G - immunology; Immunoglobulin M; Immunoglobulin M - immunology; L-Lactate dehydrogenase; L-Lactate Dehydrogenase - blood; Lactate dehydrogenase; Lactic acid; Lymphocytes; Male; Middle Aged; multidisciplinary; N protein; Nucleocapsid Proteins - immunology; Pandemics; Phosphoproteins; Pneumonia, Viral - immunology; Protein Array Analysis - methods; Proteins; Proteome - analysis; Proteome - immunology; Proteomes; SARS-CoV-2; Science; Science (multidisciplinary); Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - immunology; Vaccination; Viral Nonstructural Proteins - immunology

We still know very little about how the human immune system responds to SARS-CoV-2. Here we construct a SARS-CoV-2 proteome microarray containing 18 out of the 28 predicted proteins and apply it to the characterization of the IgG and IgM antibodies responses in the sera from 29 convalescent patients...