The fluorination effect of fluoroamphiphiles in cytosolic protein delivery

• Published in: Nature communications Vol. 9; no. 1; pp. 1377 - 8
• Main Authors: Zhang, Zhenjing, Shen, Wanwan, Ling, Jing, Yan, Yang, Hu, Jingjing, Cheng, Yiyun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 10.04.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 13/109; 14/19; 631/61/2297; 631/61/54/1754; 631/61/54/2295; Alkanes - chemistry; Alkanes - metabolism; Animals; Assembly; Biopolymer denaturation; Cycloparaffins - chemistry; Cycloparaffins - metabolism; Cytosol - metabolism; Cytotoxicity; Drug Compounding - methods; Drug Delivery Systems - methods; Endocytosis; Fluorescein-5-isothiocyanate - analogs & derivatives; Fluorescein-5-isothiocyanate - analysis; Fluorination; Gene transfer; Halogenation; HEK293 Cells; HeLa Cells; Humanities and Social Sciences; Humans; Kinetics; Mice; multidisciplinary; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; NIH 3T3 Cells; Peptides - chemical synthesis; Peptides - metabolism; Polymers; Protein denaturation; Proteins; Science; Science (multidisciplinary); Serum Albumin, Bovine - analysis; Structure-Activity Relationship; Surface-Active Agents - chemistry; Surface-Active Agents - metabolism; Toxicity

Direct delivery of proteins into cells avoids many drawbacks of gene delivery, and thus has emerging applications in biotherapy. However, it remains a challenging task owing to limited charges and relatively large size of proteins. Here, we report an efficient protein delivery system via the co-asse...