Modular enzyme assembly for enhanced cascade biocatalysis and metabolic flux

• Published in: Nature communications Vol. 10; no. 1; pp. 4248 - 11
• Main Authors: Kang, Wei, Ma, Tian, Liu, Min, Qu, Jiale, Liu, Zhenjun, Zhang, Huawei, Shi, Bin, Fu, Shuai, Ma, Juncai, Lai, Louis Tung Faat, He, Sicong, Qu, Jianan, Wing-Ngor Au, Shannon, Ho Kang, Byung, Yu Lau, Wilson Chun, Deng, Zixin, Xia, Jiang, Liu, Tiangang
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.09.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 14/28; 38/1; 38/35; 38/39; 631/61/252; 631/61/318; 631/92/552; 82/58; 82/80; 82/83; Assembling; Biocatalysis; Bioreactors - microbiology; Biosynthetic Pathways - genetics; Carotenoids; Carotenoids - metabolism; Cascade chemical reactions; Catalysis; E coli; Enzymes; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - metabolism; Humanities and Social Sciences; Industrial engineering; Lycopene; Lycopene - metabolism; Manufacturing engineering; Menaquinones; Metabolic Engineering - methods; Metabolic flux; Metabolism; Metabolites; Mevalonate pathway; Mevalonic acid; Mevalonic Acid - metabolism; multidisciplinary; Multienzyme complexes; Nanoparticles; Protein Engineering - methods; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Science; Science (multidisciplinary); Stoichiometry; Vitamin K 2 - metabolism

Enzymatic reactions in living cells are highly dynamic but simultaneously tightly regulated. Enzyme engineers seek to construct multienzyme complexes to prevent intermediate diffusion, to improve product yield, and to control the flux of metabolites. Here we choose a pair of short peptide tags (RIAD...