CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus

• Published in: Scientific reports Vol. 7; no. 1; pp. 7489 - 13
• Main Authors: Yang, Hong, Wu, Jia-Jing, Tang, Ting, Liu, Ke-De, Dai, Cheng
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.08.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 631/449/447/2311; 631/61/447; Agrobacterium - genetics; Alleles; Amino Acid Sequence; Base Sequence; Biotechnology; Brassica napus; Brassica napus - genetics; Brassica napus - metabolism; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR; CRISPR-Associated Protein 9 - genetics; CRISPR-Associated Protein 9 - metabolism; CRISPR-Cas Systems; Gene Editing - methods; Genes; Genes, Plant; Genetic Loci; Genome editing; Genomes; Genotype; Heritability; Heterozygotes; Homozygote; Homozygotes; Humanities and Social Sciences; multidisciplinary; Mutation; Mutation Rate; Oilseed crops; Oilseeds; Phenotype; Plant species; Rape plants; RNA, Guide, CRISPR-Cas Systems - genetics; RNA, Guide, CRISPR-Cas Systems - metabolism; Science; Science (multidisciplinary); Sequence Alignment; Tetraploidy

CRISPR/Cas9 is a valuable tool for both basic and applied research that has been widely applied to different plant species. Nonetheless, a systematical assessment of the efficiency of this method is not available for the allotetraploid Brassica napus —an important oilseed crop. In this study, we exa...