Lysosomal integral membrane protein-2 (LIMP-2/SCARB2) is involved in lysosomal cholesterol export

• Published in: Nature communications Vol. 10; no. 1; pp. 3521 - 12
• Main Authors: Heybrock, Saskia, Kanerva, Kristiina, Meng, Ying, Ing, Chris, Liang, Anna, Xiong, Zi-Jian, Weng, Xialian, Ah Kim, Young, Collins, Richard, Trimble, William, Pomès, Régis, Privé, Gilbert G., Annaert, Wim, Schwake, Michael, Heeren, Joerg, Lüllmann-Rauch, Renate, Grinstein, Sergio, Ikonen, Elina, Saftig, Paul, Neculai, Dante
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.08.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 14; 14/19; 14/28; 14/34; 14/35; 14/63; 631/45/287/1197; 631/80/642/1624; 64; 64/60; Animals; Carrier Proteins - genetics; Carrier Proteins - metabolism; CD36 Antigens - genetics; CD36 Antigens - metabolism; CHO Cells; Cholesterol; Cholesterol, LDL - metabolism; Cricetulus; Crosslinking; Depletion; Exports; Fibroblasts; Gene Knockout Techniques; HeLa Cells; Humanities and Social Sciences; Humans; Inclusions; Integral membrane proteins; Integrals; Intracellular Signaling Peptides and Proteins; Lipid Droplets - metabolism; Lipids; Low density lipoprotein; Lysosomal Membrane Proteins - genetics; Lysosomal Membrane Proteins - metabolism; Lysosomes; Lysosomes - metabolism; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mice; Molecular modelling; multidisciplinary; Niemann-Pick C1 Protein; Protein Domains; Proteins; Receptors, Scavenger - genetics; Receptors, Scavenger - metabolism; RNA, Small Interfering - metabolism; Science; Science (multidisciplinary); Sterol regulatory element-binding protein; Thermophoresis; Transport

The intracellular transport of cholesterol is subject to tight regulation. The structure of the lysosomal integral membrane protein type 2 (LIMP-2, also known as SCARB2) reveals a large cavity that traverses the molecule and resembles the cavity in SR-B1 that mediates lipid transfer. The detection o...