rBMSCs/ F92A-Cav1对PAH大鼠的治疗作用及其机制
目的 探讨过表达F92A-丙氨酸替代窖蛋白-1（Cav1）的大鼠骨髓间充质干细胞（rBMSCs/ F92A-Cav1）对肺动脉高压（PAH）大鼠的治疗作用及其机制.方法 予成年雄性Wistar大鼠腹腔注射1%野百合碱（MCT,60 mg/kg）建立PAH模型,建模后2周随机分为3组（10只/组）：PAH 组 （仅给予MCT）、Cav1 组 （转导LV-Cav1的rBMSCs）、F92A-Cav1 组（转导LV-F92A-Cav1的rBMSCs）,同时设置正常组.基因修饰的rBMSCs（1×106/mL）于尾静脉移植入各组PAH大鼠,移植后3周,采用Image-Pro Plus 6 softwa...
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| Published in | 山东医药 Vol. 57; no. 13; pp. 20 - 23 |
|---|---|
| Main Author | |
| Format | Magazine Article |
| Language | Chinese |
| Published |
聊城市人民医院,山东聊城,252000%聊城市人民医院,山东聊城252000
2017
查尔斯特大学生物医学院 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-266X |
| DOI | 10.3969/j.issn.1002-266X.2017.13.006 |
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| Abstract | 目的 探讨过表达F92A-丙氨酸替代窖蛋白-1（Cav1）的大鼠骨髓间充质干细胞（rBMSCs/ F92A-Cav1）对肺动脉高压（PAH）大鼠的治疗作用及其机制.方法 予成年雄性Wistar大鼠腹腔注射1%野百合碱（MCT,60 mg/kg）建立PAH模型,建模后2周随机分为3组（10只/组）：PAH 组 （仅给予MCT）、Cav1 组 （转导LV-Cav1的rBMSCs）、F92A-Cav1 组（转导LV-F92A-Cav1的rBMSCs）,同时设置正常组.基因修饰的rBMSCs（1×106/mL）于尾静脉移植入各组PAH大鼠,移植后3周,采用Image-Pro Plus 6 software评估肺动脉中膜厚度指数（MT%）、右心肥大指数（RVHI）,采用Griess法检测血清NO水平,采用Western blotting法检测肺组织PI3K、AKT蛋白的相对表达量.结果 PAH组MT%、RVHI及肺组织PI3K、AKT蛋白相对表达量均较正常组升高,但血清NO水平降低（P＜0.05或＜0.01）;与PAH组相比,Cav1组、F92A-Cav1组中MT%、RVHI及肺组织PI3K、AKT蛋白相对表达量均降低,而血清NO水平增加,以F92A-Cav1组为著（P＜0.05或＜0.01）.结论 rBMSCs/F92A-Cav1可通过解除野生型Cav1对eNOS的抑制,促进NO释放,从而抑制PAH大鼠肺组织PI3K/AKT通路激活,进而发挥对PAH大鼠的治疗作用. |
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| AbstractList | R563.9; 目的 探讨过表达F92A-丙氨酸替代窖蛋白-1(Cav1)的大鼠骨髓间充质干细胞(rBMSCs/ F92A-Cav1)对肺动脉高压(PAH)大鼠的治疗作用及其机制.方法 予成年雄性Wistar大鼠腹腔注射1%野百合碱(MCT,60 mg/kg)建立PAH模型,建模后2周随机分为3组(10只/组):PAH 组 (仅给予MCT)、Cav1 组 (转导LV-Cav1的rBMSCs)、F92A-Cav1 组(转导LV-F92A-Cav1的rBMSCs),同时设置正常组.基因修饰的rBMSCs(1×106/mL)于尾静脉移植入各组PAH大鼠,移植后3周,采用Image-Pro Plus 6 software评估肺动脉中膜厚度指数(MT%)、右心肥大指数(RVHI),采用Griess法检测血清NO水平,采用Western blotting法检测肺组织PI3K、AKT蛋白的相对表达量.结果 PAH组MT%、RVHI及肺组织PI3K、AKT蛋白相对表达量均较正常组升高,但血清NO水平降低(P＜0.05或＜0.01);与PAH组相比,Cav1组、F92A-Cav1组中MT%、RVHI及肺组织PI3K、AKT蛋白相对表达量均降低,而血清NO水平增加,以F92A-Cav1组为著(P＜0.05或＜0.01).结论 rBMSCs/F92A-Cav1可通过解除野生型Cav1对eNOS的抑制,促进NO释放,从而抑制PAH大鼠肺组织PI3K/AKT通路激活,进而发挥对PAH大鼠的治疗作用. 目的 探讨过表达F92A-丙氨酸替代窖蛋白-1（Cav1）的大鼠骨髓间充质干细胞（rBMSCs/ F92A-Cav1）对肺动脉高压（PAH）大鼠的治疗作用及其机制.方法 予成年雄性Wistar大鼠腹腔注射1%野百合碱（MCT,60 mg/kg）建立PAH模型,建模后2周随机分为3组（10只/组）：PAH 组 （仅给予MCT）、Cav1 组 （转导LV-Cav1的rBMSCs）、F92A-Cav1 组（转导LV-F92A-Cav1的rBMSCs）,同时设置正常组.基因修饰的rBMSCs（1×106/mL）于尾静脉移植入各组PAH大鼠,移植后3周,采用Image-Pro Plus 6 software评估肺动脉中膜厚度指数（MT%）、右心肥大指数（RVHI）,采用Griess法检测血清NO水平,采用Western blotting法检测肺组织PI3K、AKT蛋白的相对表达量.结果 PAH组MT%、RVHI及肺组织PI3K、AKT蛋白相对表达量均较正常组升高,但血清NO水平降低（P＜0.05或＜0.01）;与PAH组相比,Cav1组、F92A-Cav1组中MT%、RVHI及肺组织PI3K、AKT蛋白相对表达量均降低,而血清NO水平增加,以F92A-Cav1组为著（P＜0.05或＜0.01）.结论 rBMSCs/F92A-Cav1可通过解除野生型Cav1对eNOS的抑制,促进NO释放,从而抑制PAH大鼠肺组织PI3K/AKT通路激活,进而发挥对PAH大鼠的治疗作用. |
| Abstract_FL | To investigate the therapeutic effect and mechanism of Caveolin-1 (Cav1) mutant to F92A-Cav1 modified rat bone marrow mesenchymal stem cells (rBMSCs/F92A-Cav1) on rats with pulmonary hypertension (PAH).Methods PAH was induced by intraperitoneal injection of 1% monocrotaline (MCT, 60 mg/kg) in adult male Wistar rats.The PAH rats were randomly divided into four groups (10 rats/group) after 2 weeks of MCT injection: PAH group (only MCT), Cav1 group (transduced with LV-Cav1 modified rBMSCs), F92A-Cav1 group (transduced with LV-F92A-Cav1modified rBMSCs), and the control group.Gene modified rBMSCs (1×106/ml) were transplanted into PAH rats in each group by tail vein injection.After 3 weeks of transplantation, the percentage of media wall thickness (MT%) and right ventricular hypertrophy index (RVHI) were evaluated by Image-Pro Plus 6 software, serum NO concentration was checked by Griess method, and the expression of phosphoinositide 3-kinase(PI3K) and protein kinase B (AKT) was detected by Western blotting.Results MT%, RVHI, PI3K and AKT was all increased but NO was decreased in the PAH group as compared with that of the control group(P<0.05 or P<0.01).Compared with the PAH group, MT%, RVHI, PI3K and AKT was decreased but NO was increased in the Cav1 and F92A-Cav1 groups, especially in the F92A-Cav1 group (P<0.01).Conclusion F92A-Cav1 can abrogate the inhibitory effect of wild-type Cav1 on eNOS and promote the release of NO, then inhibit the activation of PI3K/Akt signaling pathway and thus exert its therapeutic effect on PAH rats. |
| Author | 徐聪 夏鹏 杨红莉 唐文强 潘丽 王兰花 陈双峰 张颖新 王乐信 陈海英 |
| AuthorAffiliation | 聊城市人民医院,山东聊城252000 查尔斯特大学生物医学院 |
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| Author_FL | TANG Wenqiang WANG Lexin YANG Hongli WANG Lanhua CHEN Haiying XU Cong PAN Li XIA Peng CHEN Shuangfeng ZHANG Yingxin |
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| Keywords | Caveolin-1 大鼠 窖蛋白-1 mesenchymal stem cells 肺动脉高压 骨髓间充质干细胞 rats pulmonary arterial hypertension |
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| Notes | To investigate the therapeutic effect and mechanism of Caveolin-1 （Cav1） mutant to F92A-Cav1 modified rat bone marrow mesenchymal stem cells （rBMSCs/F92A-Cav1） on rats with pulmonary hypertension （PAH）.Methods PAH was induced by intraperitoneal injection of 1% monocrotaline （MCT, 60 mg/kg） in adult male Wistar rats.The PAH rats were randomly divided into four groups （10 rats/group） after 2 weeks of MCT injection： PAH group （only MCT）, Cav1 group （transduced with LV-Cav1 modified rBMSCs）, F92A-Cav1 group （transduced with LV-F92A-Cav1modified rBMSCs）, and the control group.Gene modified rBMSCs （1×106/ml） were transplanted into PAH rats in each group by tail vein injection.After 3 weeks of transplantation, the percentage of media wall thickness （MT%） and right ventricular hypertrophy index （RVHI） were evaluated by Image-Pro Plus 6 software, serum NO concentration was checked by Griess method, and the expression of phosphoinositide 3-kinase（PI3K） and protein kinase B （AKT） was detected by Western blotting.Results |
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| Publisher | 聊城市人民医院,山东聊城,252000%聊城市人民医院,山东聊城252000 查尔斯特大学生物医学院 |
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| Snippet | 目的 探讨过表达F92A-丙氨酸替代窖蛋白-1（Cav1）的大鼠骨髓间充质干细胞（rBMSCs/ F92A-Cav1）对肺动脉高压（PAH）大鼠的治疗作用及其机制.方法 予成年雄性Wistar大鼠腹腔注射1%野百合碱（MCT,60 mg/kg）建立PAH模型,建模后2周随机分为3组（10只/组）：PAH 组... R563.9; 目的 探讨过表达F92A-丙氨酸替代窖蛋白-1(Cav1)的大鼠骨髓间充质干细胞(rBMSCs/ F92A-Cav1)对肺动脉高压(PAH)大鼠的治疗作用及其机制.方法 予成年雄性Wistar大鼠腹腔注射1%野百合碱(MCT,60... |
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| SubjectTerms | 大鼠 窖蛋白-1 肺动脉高压 骨髓间充质干细胞 |
| Title | rBMSCs/ F92A-Cav1对PAH大鼠的治疗作用及其机制 |
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