Conformational dynamics of ligand-dependent alternating access in LeuT

• Published in: Nature structural & molecular biology Vol. 21; no. 5; pp. 472 - 479
• Main Authors: Kazmier, Kelli, Sharma, Shruti, Quick, Matthias, Islam, Shahidul M, Roux, Benoît, Weinstein, Harel, Javitch, Jonathan A, Mchaourab, Hassane S
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.05.2014; Nature Publishing Group
• Subjects: 631/535; 631/57/2272; 82; Bacterial proteins; Bacterial Proteins - chemistry; Bacterial Proteins - physiology; Biochemistry; Biological Microscopy; Catalysis; Cell research; Crystal structure; Ion transport; Life Sciences; Ligands; Membrane Biology; Models, Molecular; Molecular biology; Neural transmission; Neurotransmitter Transport Proteins - chemistry; Neurotransmitters; Physiological aspects; Protein Structure; Protein Structure, Tertiary; Sodium; Spin Labels; Structural models; Symporters - chemistry; Symporters - physiology; Testing

LeuT is a Na + -coupled amino acid transporter that is similar in sequence and function to eukaryotic neurotransmitter/sodium symporters, which are active in reuptake of neurotransmitters from the synapse. Distance measurements between spin-label pairs are used to identify ligand-dependent structura...