Immunohistochemical and molecular analysis of Pasteurella multocida in a rabbit with suppurative pleuropneumonia

A 1-month-old rabbit, imported as a pet by a distributor, died suddenly in the quarantine period in Japan due to suppurative pleuropneumonia. A bacterial isolate from its right lung was identified as Pasteurella multocida serotype A: 11. The isolate was classified as ST204 using the RIRDC scheme of...

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Published inJournal of Veterinary Medical Science Vol. 82; no. 1; pp. 89 - 93
Main Authors UENOYAMA, Kei, UENO, Yuichi, TOSAKI, Kaori, ABETO, Yusuke, ITO, Hirotaka, KATSUDA, Ken, SHIBAHARA, Tomoyuki
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.01.2020
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
Subjects
immunohistochemical assay
Multilocus sequence typing
Pasteurella multocida
Pasteurellosis
Pathology
Pleuropneumonia
rabbit
Serotyping
suppurative pleuropneumonia
immunohistochemical assay
multilocus sequence typing
rabbit
suppurative pleuropneumonia
Pasteurella multocida
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Summary:A 1-month-old rabbit, imported as a pet by a distributor, died suddenly in the quarantine period in Japan due to suppurative pleuropneumonia. A bacterial isolate from its right lung was identified as Pasteurella multocida serotype A: 11. The isolate was classified as ST204 using the RIRDC scheme of multilocus sequence typing, suggesting that the isolate was genetically related to European isolates of the same sequence type listed in the PubMLST database and not to four other isolates that originated from past imported rabbits. In the immunohistochemical assay, an antiserum recognizing the somatic serotype 11 antigen generated from chicken could specifically detect P. multocida, indicating that the antiserum for somatic serotyping was useful for immunohistochemical diagnosis of rabbit pasteurellosis.
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These authors contributed equally to this work.
ISSN:0916-7250
1347-7439
1347-7439
DOI:10.1292/jvms.19-0561