Dual Binding of Chromomethylase Domains to H3K9me2-Containing Nucleosomes Directs DNA Methylation in Plants

• Published in: Cell Vol. 151; no. 1; pp. 167 - 180
• Main Authors: Du, Jiamu, Zhong, Xuehua, Bernatavichute, Yana V., Stroud, Hume, Feng, Suhua, Caro, Elena, Vashisht, Ajay A., Terragni, Jolyon, Chin, Hang Gyeong, Tu, Andy, Hetzel, Jonathan, Wohlschlegel, James A., Pradhan, Sriharsa, Patel, Dinshaw J., Jacobsen, Steven E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 28.09.2012
• Subjects: Amino Acid Sequence; Arabidopsis - genetics; Arabidopsis - metabolism; corn; crystal structure; Crystallography, X-Ray; DNA (Cytosine-5-)-Methyltransferases - chemistry; DNA (Cytosine-5-)-Methyltransferases - metabolism; DNA Methylation; DNA, Plant - metabolism; epigenetics; gene expression; genome; Heterochromatin - metabolism; histones; Histones - metabolism; methylation; Models, Molecular; Molecular Sequence Data; mutation; nucleosomes; Nucleosomes - metabolism; peptides; Sequence Alignment; Zea mays - genetics; Zea mays - metabolism

DNA methylation and histone modification exert epigenetic control over gene expression. CHG methylation by CHROMOMETHYLASE3 (CMT3) depends on histone H3K9 dimethylation (H3K9me2), but the mechanism underlying this relationship is poorly understood. Here, we report multiple lines of evidence that CMT...