Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry

• Published in: Biosensors & bioelectronics Vol. 86; pp. 420 - 425
• Main Authors: Tian, Bo, Qiu, Zhen, Ma, Jing, Zardán Gómez de la Torre, Teresa, Johansson, Christer, Svedlindh, Peter, Strömberg, Mattias
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.12.2016
• Subjects: AC susceptometer; AC susceptometry; agar gel electrophoresis; Amplification; bioassay; Brownian relaxation; Brownian relaxations; Chemical reactions; cross reaction; DNA, Viral - analysis; DNA, Viral - genetics; Equipment Design; Equipment Failure Analysis; Fluid dynamics; Fluid flow; Homogeneous detections; human; Hydrodynamic volumes; Hydrodynamics; incubation time; infection control; Isotherms; Lamps; limit of detection; Loop mediated isothermal amplifications; Loop-mediated isothermal amplification; Magnetic nano-particles; magnetic nanoparticle; Magnetic nanoparticles; Magnetometry - instrumentation; Metal Nanoparticles - chemistry; Microchemistry - instrumentation; Nanomagnetics; Nanoparticles; Nucleic Acid Amplification Techniques - instrumentation; nucleotide sequence; Oligonucleotide detection; Oligonucleotides; Polymerase chain reaction; process optimization; Proteins; Reproducibility of Results; Reverse transcription-polymerase chain reaction; RNA extraction; scanning electron microscopy; Sensitivity and Specificity; Sequence Analysis, DNA - instrumentation; serology; virus detection; virus load; Viruses; Windows (intervals); Zika fever; Zika virus; Zika Virus - genetics; Zika Virus - isolation & purification; Loop-mediated isothermal amplification; Zika virus; AC susceptometer; Brownian relaxation; Magnetic nanoparticles

Because of the serological cross-reactivity among the flaviviruses, molecular detection methods, such as reverse-transcription polymerase chain reaction (RT-PCR), play an important role in the recent Zika outbreak. However, due to the limited sensitivity, the detection window of RT-PCR for Zika vire...