Genome-Scale Metabolic Network Validation of Shewanella oneidensis Using Transposon Insertion Frequency Analysis

• Published in: PLoS computational biology Vol. 10; no. 9; p. e1003848
• Main Authors: Yang, Hong, Krumholz, Elias W., Brutinel, Evan D., Palani, Nagendra P., Sadowsky, Michael J., Odlyzko, Andrew M., Gralnick, Jeffrey A., Libourel, Igor G. L.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.09.2014; Public Library of Science (PLoS)
• Subjects: Agreements; Automation; Binding sites; Biology and Life Sciences; Deoxyribonucleic acid; DNA; DNA Transposable Elements - genetics; DNA, Bacterial - analysis; DNA, Bacterial - genetics; Gene mutations; Gene Regulatory Networks - genetics; Genes; Genetic aspects; Genetic testing; Genome, Bacterial - genetics; Genomes; Genomics; High-Throughput Nucleotide Sequencing - methods; Metabolic Networks and Pathways - genetics; Metabolism; Metabolites; Methods; Mutagenesis; Mutagenesis, Site-Directed; Mutation; Physiological aspects; Proteins; Shewanella; Shewanella - genetics; Shewanella - metabolism; Transposons; United States

Transposon mutagenesis, in combination with parallel sequencing, is becoming a powerful tool for en-masse mutant analysis. A probability generating function was used to explain observed miniHimar transposon insertion patterns, and gene essentiality calls were made by transposon insertion frequency a...