农杆菌介导EPSPS抗草甘膦基因转化马铃薯品种

摘要：以马铃薯品种费乌瑞它微型薯为外植体，利用农杆菌介导法，将EPSPS抗草甘膦基因转入马铃薯，并对其遗传转化的影响因素进行研究。经PPT筛选获得抗性植株17株；经PCR检测，17株呈阳性；RT—PCR检测，6株呈阳性，说明肼峪P5基因能够正常转录；Southernblot杂交分析，5株呈阳性，证明E朋PS基因已整合到马铃薯基因组中。对转基因植株后代进行抗草甘膦试验，转基因材料对浓度0．4g·L-1的草甘膦表现出抗性。...

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Published in东北农业大学学报 Vol. 44; no. 10; pp. 18 - 24
Main Author 卢翠华 王石 张丽莉 曹士亮 姜丽丽 张欢 潘映雪 林忠平
Format Journal Article
LanguageChinese
Published 东北农业大学农学院,哈尔滨,150030%黑龙江省农业科学院玉米研究所,哈尔滨,150086%黑龙江八一农垦大学农学院,黑龙江大庆,163000%北京大学生命科学学院,北京,100871 2013
Subjects
EPSPS基因
农杆菌介导
草甘膦
遗传转化
马铃薯
EPSPS基因
农杆菌介导
Agrobacterium
遗传转化
potato
genetic transformation
草甘膦
EPSPS gene
马铃薯
glyphosate
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ISSN1005-9369

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Summary:摘要：以马铃薯品种费乌瑞它微型薯为外植体，利用农杆菌介导法，将EPSPS抗草甘膦基因转入马铃薯，并对其遗传转化的影响因素进行研究。经PPT筛选获得抗性植株17株；经PCR检测，17株呈阳性；RT—PCR检测，6株呈阳性，说明肼峪P5基因能够正常转录；Southernblot杂交分析，5株呈阳性，证明E朋PS基因已整合到马铃薯基因组中。对转基因植株后代进行抗草甘膦试验，转基因材料对浓度0．4g·L-1的草甘膦表现出抗性。
Bibliography:23-1391/S
In this study, EPSPS gene was transferred into the potato （Solanum tuberosum L.） cultivars, ＇Favorite＇ by Agrobacterium-mediated transformation, and influence factors of genetic trans- formation were optimized. Microtuber discs were used as explants. Resistant plants were analyzed by PCR detection, of which 17 plants were positive. By RT-PCR test, six strains of ＇Favorite＇ were positive; EPSPS gene could be transcribed nomally. And these resistant plants were analyzed by Southern blot, of which five plants had a positive amplification. Southern blot showed that single copies of the EPSPS gene were inserted into the genome of the transgenic plants. By the result of glyphosate resistant experiment, the transgenic materials showed resistance to glyphosate.
potato; Agrobacterium; EPSPS gene; genetic transformation; glyphosate
LU Cuihua1, WANG Shi＇, ZHANG Lili＇, CAO Shiliang2, JIANG Lili3, ZHANG Huan~, PAN Yingxue~, LIN Zhongping＇（l. School of Agriculture, Northeast Agricultural University, Harbin 150030, C
ISSN:1005-9369