Fosfomycin Resistance in Avian Pathogenic Escherichia coil Isolates

• Published in: 农业科学学报（英文版） Vol. 11; no. 12; pp. 2051 - 2057
• Main Author: JIN Wen-jie ZHENG Zhi-ming WANG Qian-qian QIN Ai-jian SHAO Hong-xia QIAN Kun
• Format: Journal Article
• Language: English
• Published: Animal Disease Preventive and Control Center of Yangzhou, Yangzhou 225009, P.R.China 2012; Key Lab for Avian Preventive Medicine, Ministry of Education/College of Veterinary Medicine, Yangzhou University, Yangzhou 225009,P.R.China%Key Lab for Avian Preventive Medicine, Ministry of Education/College of Veterinary Medicine, Yangzhou University, Yangzhou 225009,P.R.China; Circulation Industry Promotion Center, Ministry of Commerce, Beijing 100070, P.R.China%Key Lab for Avian Preventive Medicine, Ministry of Education/College of Veterinary Medicine, Yangzhou University, Yangzhou 225009,P.R.China
• Subjects: APEC; 最低抑菌浓度; 检测试验; 磷霉素; 禽流感; 禽致病性大肠杆菌; 耐药机制; 菌株; avian pathogenic Escherichia coli (APEC); fosfomycin; resistance
• ISSN: 2095-3119

Fosfomycin, a broad-spectrum antibiotic against both Gram-positive and Gram-negative bacteria, is very important in the clinic but many fosfomycin-resistant bacteria have been isolated from patients. In this study, the resistance mechanism of three fosfomycin-resistant avian pathogenic Escherichia coli （APEC） strains （JE1, IF7 and CD11） isolated from septicemic chickens were analyzed. The results showed that their fosfomycin-resistance mechanisms were different. An alteration in the glpT transport system was the main reason of the fosfomycin-resistance mechanisms of strain IF7. Compared with the control stain BL21, the capacity of fosfomycin-uptake was low in all these three stains （JE 1 〉IFT〉CD 11）. Sequence results of murA showed that there were more than 10 sites of nucleotide mutation, but only one amino acid mutation T116A showed in CD11. Real-time detection test showed that the expression level of the murA gene of the three stains was significantly increased （four times increase in strain CD11 and two times increase in strains JE1 and IFT）. The transformation and recombinant test showed that the recombinant bacteria with the murA of JE1 and CD11 showed high minimal inhibitory concentration （MIC） against fosfomycin. From the results of this research, it showed that most of the fosfomycin- resistance mechanisms once showed in patient bacteria have appeared in the APEC strains and the fosfomycin-resistance mechanism of the three APEC isolates was different.