Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion

• Published in: Scientific reports Vol. 10; no. 1; p. 7402
• Main Authors: Frausto, Ricardo F., Swamy, Vinay S., Peh, Gary S. L., Boere, Payton M., Hanser, E. Maryam, Chung, Doug. D., George, Benjamin L., Morselli, Marco, Kao, Liyo, Azimov, Rustam, Wu, Jessica, Pellegrini, Matteo, Kurtz, Ira, Mehta, Jodhbir S., Aldave, Anthony J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.05.2020; Nature Publishing Group
• Subjects: 631/114; 631/337/2019; 631/80; 631/80/509; 631/80/79; Adolescent; Adult; BASIC BIOLOGICAL SCIENCES; Cell adhesion; Cell biology; Cell culture; Cell Culture Techniques - methods; Cell Movement; Cell Proliferation; Cellular Senescence; Child; Child, Preschool; Computational Biology; Computational biology and bioinformatics; Cornea; Corneal Transplantation; Endothelial cells; Endothelium; Endothelium, Corneal - cytology; Female; Humanities and Social Sciences; Humans; Male; multidisciplinary; Phenotype; Phenotypes; Science; Science & Technology - Other Topics; Science (multidisciplinary); Senescence; Signal Transduction; Transcriptome; Transcriptomics; Young Adult

The advent of cell culture-based methods for the establishment and expansion of human corneal endothelial cells (CEnC) has provided a source of transplantable corneal endothelium, with a significant potential to challenge the one donor-one recipient paradigm. However, concerns over cell identity rem...