Knockdown of leptin A expression dramatically alters zebrafish development

• Published in: General and comparative endocrinology Vol. 178; no. 3; pp. 562 - 572
• Main Authors: Liu, Qin, Dalman, Mark, Chen, Yun, Akhter, Mashal, Brahmandam, Sravya, Patel, Yesha, Lowe, Josef, Thakkar, Mitesh, Gregory, Akil-Vuai, Phelps, Daryllanae, Riley, Caitlin, Londraville, Richard L
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.2012
• Subjects: Animals; Auditory; Bone; brain; Cell Differentiation - genetics; Cell Differentiation - physiology; Central nervous system; Danio rerio; Differentiation; early development; ears; Embryo, Nonmammalian - metabolism; Endocrinology & Metabolism; eyes; fish development; Gene Expression Regulation, Developmental - physiology; larvae; leptin; Leptin - genetics; Leptin - metabolism; leptin receptors; mammals; Metabolism; oligonucleotides; Oligonucleotides, Antisense; pericardium; phenotype; Signal Transduction - genetics; Signal Transduction - physiology; tail; Visual; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - metabolism; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism; Auditory; Bone; Differentiation; Metabolism; Visual; Central nervous system
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2012.07.011

Graphical abstract Zebrafish embryos 6 days post fertilization. Embryo on right was injected with an anti-leptin morpholino oligonucleotide within 20 min of fertilization. Sb = swim bladder, pc = pericardium, tr = trunk. Highlights ► Antisense morpholino oligonucleotides reduce leptin signaling in zebrafish embryos. ► Morphants have large yolk, small eyes, kinked tails, and much reduced semicircular canals. ► The same phenotype results from knocking down its receptor. The knockdown can be rescued via co-injection with recombinant zebrafish leptin. ► Leptin signaling is required for normal development in zebrafish embryos.