Human Primary Cell-Based Organotypic Microtissues for Modeling Small Intestinal Drug Absorption

• Published in: Pharmaceutical research Vol. 35; no. 4; pp. 72 - 18
• Main Authors: Ayehunie, Seyoum, Landry, Tim, Stevens, Zachary, Armento, Alex, Hayden, Patrick, Klausner, Mitchell
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2018; Springer; Springer Nature B.V
• Subjects: Administration, Oral; Adult; Bioavailability; Biochemistry; Biological Availability; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Caco-2 Cells; Cells; Drug Evaluation, Preclinical - methods; Drug interaction; Drug Interactions; Enterocytes; Enzymes; Epithelial Cells; Female; Fibroblasts; Gastrointestinal agents; Humans; Intestinal Absorption; Intestinal Mucosa - cytology; Intestinal Mucosa - metabolism; Intestine, Small - cytology; Intestine, Small - metabolism; Medical Law; Oral administration; Permeability; Pharmacology/Toxicology; Pharmacy; Physiological aspects; Primary Cell Culture; Research Paper; Small intestine; Tissue Culture Techniques - methods; Young Adult; drug permeation; drug transporters; caco-2; organotypic small intestinal microtissues; drug-drug interaction; drug metabolizing enzymes

Purpose The study evaluates the use of new in vitro primary human cell-based organotypic small intestinal (SMI) microtissues for predicting intestinal drug absorption and drug-drug interaction. Methods The SMI microtissues were reconstructed using human intestinal fibroblasts and enterocytes culture...