Nucleofection induces transient eIF2α phosphorylation by GCN2 and PERK

• Published in: Gene therapy Vol. 20; no. 2; pp. 136 - 142
• Main Authors: Anderson, B R, Karikó, K, Weissman, D
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.02.2013; Nature Publishing Group
• Subjects: 631/154/51/391; 631/1647/2300; 631/250/516; Animals; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cell Biology; Cell Line; Deoxyribonucleic acid; DNA; DNA binding proteins; Drug development; eIF-2 kinase; eIF-2 Kinase - genetics; eIF-2 Kinase - metabolism; Endoplasmic reticulum; Eukaryotic Initiation Factor-2 - genetics; Eukaryotic Initiation Factor-2 - metabolism; Gene Expression; Gene Therapy; Human Genetics; Humans; Initiation factor eIF-2; Initiation factor eIF-2α; Kinases; Mammalian cells; Messenger RNA; Mice; mRNA; Nanotechnology; nucleic acids; original-article; Phosphorylation; Physiological aspects; Protein kinase; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; siRNA; Transfection; Transfection - methods; Translation; United States; eIF2α; GCN2; mRNA therapy; PERK; nucleofection

Nucleofection permits efficient transfection even with difficult cell types such as primary and non-dividing cells, and is used to deliver various nucleic acids, including DNA, mRNA, and small interfering RNA. Unlike DNA and small interfering RNA, mRNA is subject to rapid degradation, which necessit...