OB protein binds specifically to the choroid plexus of mice and rats

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 93; no. 11; pp. 5668 - 5673
• Main Authors: Devos, R. (Roche Research Gent, Gent, Belgium.), Richards, J.G, Campfield, L.A, Tartaglia, L.A, Guisez, Y, Heyden, J. van der, Travernier, J, Plaetinck, G, Burn, P
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 28.05.1996; National Acad Sciences; National Academy of Sciences
• Subjects: Adipose tissues; Alkaline Phosphatase - metabolism; Animals; Autoradiography; Binding Sites; Biochemistry; Body weight; Brain - metabolism; CHIMIE; Choroid plexus; Choroid Plexus - metabolism; Food intake; Genes; Genetic mutation; HORMONAS; HORMONE; Iodine Radioisotopes; Leptin; MENINGE; MENINGES; Mice; Mice, Inbred C57BL; Mice, Obese; Obesity; Obesity - metabolism; Organ Specificity; PEPTIDE; PEPTIDOS; Proteins; Proteins - metabolism; QUIMICA; RADIOGRAFIA; RADIOGRAPHIE; RAT; RATA; RATON; Rats; Rats, Zucker; RECEPTEUR D'HORMONE; RECEPTORES DE HORMONAS; Receptors; Recombinant Fusion Proteins - metabolism; Recombinant Proteins - metabolism; Rodents; SOBREPESO; SOURIS; SURPOIDS; TECHNIQUE DES TRACEURS; TECNICAS DE TRAZADORES; Tritium

Binding studies were conducted to identify the anatomical location of brain target sites for OB protein, the ob gene product. 125I-labeled recombinant mouse OB protein or alkaline phosphatase-OB fusion proteins were used for in vitro and in vivo binding studies. Coronal brain sections or fresh tissu...