Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1

• Published in: eLife Vol. 6
• Main Authors: Rashid, Fahad, Harris, Paul D, Zaher, Manal S, Sobhy, Mohamed A, Joudeh, Luay I, Yan, Chunli, Piwonski, Hubert, Tsutakawa, Susan E, Ivanov, Ivaylo, Tainer, John A, Habuchi, Satoshi, Hamdan, Samir M
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 23.02.2017; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: Analysis; BASIC BIOLOGICAL SCIENCES; Biochemistry; Biophysics and Structural Biology; Deoxyribonucleic acid; DNA; DNA - metabolism; DNA binding proteins; DNA biosynthesis; DNA repair; Endonuclease; expressed proteins in E. coli; FEN1 protein; Flap Endonucleases - metabolism; Fluorescence resonance energy transfer; Genomes; Genomic instability; Genomics; Geometry; Histograms; Human purified proteins; Humans; Labeling; Life Sciences & Biomedicine - Other Topics; Models, Biological; Models, Molecular; Nuclease; Nucleases; Nucleic Acid Conformation; Protein Binding; Protein Conformation; Protein structure; recombinant protein; Recombination; Simulation; Single Molecule Imaging; Standard deviation; Substrate Specificity; Human purified proteins; recombinant protein; biochemistry; structural biology; none; biophysics; expressed proteins in E. coli
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.21884

Human flap endonuclease 1 (FEN1) and related structure-specific 5’nucleases precisely identify and incise aberrant DNA structures during replication, repair and recombination to avoid genomic instability. Yet, it is unclear how the 5’nuclease mechanisms of DNA distortion and protein ordering robustl...