pH and glucose profiles in aggregates of Bacillus laevolacticus
Size distributions and glucose and pH profiles of aggregates of the D-(--)-lactic acid-producing organism Bacillus laevolacticus were measured. The organisms were grown in continuous culture with a medium glucose concentration of either 280 or 110 mM. A maximal aggregate diameter of 2.2 mm, with a S...
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Published in | Applied and Environmental Microbiology Vol. 59; no. 8; pp. 2474 - 2478 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Washington, DC
American Society for Microbiology
01.08.1993
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Abstract | Size distributions and glucose and pH profiles of aggregates of the D-(--)-lactic acid-producing organism Bacillus laevolacticus were measured. The organisms were grown in continuous culture with a medium glucose concentration of either 280 or 110 mM. A maximal aggregate diameter of 2.2 mm, with a Sauter mean of 1.46 mm, was determined for the former culture condition, whereas aggregates from a culture with 110 mM glucose input had a maximal diameter of 1.9 mm (Sauter mean of 1.07 mm). A pH gradient of approximately 2 U was observed for large aggregates (above 1.5 mm). In smaller aggregates (0.75 mm), the pH value in the interior part was approximately 0.4 U lower than that in the culture fluid. It could be concluded that, in cultures with the high glucose input, lactic acid accumulated within the aggregates to such an extent that metabolism in the central region of the larger aggregates could not proceed further. In these cultures, approximately 90% of the total biomass was active. In aggregates from cultures with a low glucose input, glucose only partly penetrated the larger-sized aggregates, and the activity of this culture was reduced to approximately 70% of the biomass. These aggregates were found to decrease in size after prolonged periods of cultivation. It is suggested that this is caused by glucose depletion in the interior of the aggregates. It is concluded that the availability of glucose is an important factor in determining the size of aggregates of B. laevolacticus. |
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AbstractList | Size distributions and glucose and pH profiles of aggregates of the D-(-)-lactic acid-producing organism Bacillus laevolacticus were measured. The organisms were grown in continuous culture with a medium glucose concentration of either 280 or 110 mM. A maximal aggregate diameter of 2.2 mm, with a Sauter mean of 1.46 mm, was determined for the former culture condition, whereas aggregates from a culture with 110 mM glucose input had a maximal diameter of 1.9 mm (Sauter mean of 1.07 mm). A pH gradient of approximately 2 U was observed for large aggregates (above 1.5 mm). In smaller aggregates (0.75 mm), the pH value in the interior part was approximately 0.4 U lower than that in the culture fluid. It could be concluded that, in cultures with the high glucose input, lactic acid accumulated within the aggregates to such an extent that metabolism in the central region of the larger aggregates could not proceed further. In these cultures, approximately 90% of the total biomass was active. In aggregates from cultures with a low glucose input, glucose only partly penetrated the larger-sized aggregates, and the activity of this culture was reduced to approximately 70% of the biomass. These aggregates were found to decrease in size after prolonged periods of cultivation. It is suggested that this is caused by glucose depletion in the interior of the aggregates. It is concluded that the availability of glucose is an important factor in determining the size of aggregates of B. laevolacticus. Size distributions and glucose and pH profiles of aggregates of the d -(-)-lactic acid-producing organism Bacillus laevolacticus were measured. The organisms were grown in continuous culture with a medium glucose concentration of either 280 or 110 mM. A maximal aggregate diameter of 2.2 mm, with a Sauter mean of 1.46 mm, was determined for the former culture condition, whereas aggregates from a culture with 110 mM glucose input had a maximal diameter of 1.9 mm (Sauter mean of 1.07 mm). A pH gradient of approximately 2 U was observed for large aggregates (above 1.5 mm). In smaller aggregates (0.75 mm), the pH value in the interior part was approximately 0.4 U lower than that in the culture fluid. It could be concluded that, in cultures with the high glucose input, lactic acid accumulated within the aggregates to such an extent that metabolism in the central region of the larger aggregates could not proceed further. In these cultures, approximately 90% of the total biomass was active. In aggregates from cultures with a low glucose input, glucose only partly penetrated the larger-sized aggregates, and the activity of this culture was reduced to approximately 70% of the biomass. These aggregates were found to decrease in size after prolonged periods of cultivation. It is suggested that this is caused by glucose depletion in the interior of the aggregates. It is concluded that the availability of glucose is an important factor in determining the size of aggregates of B. laevolacticus. Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: AEM Size distributions and glucose and pH profiles of aggregates of the D-lactic acid-producing organism Bacillus laevolacticus were measured. It was determined that the availability of glucose is an important factor in determining the size of aggregates of B. laevolacticus. |
Author | Heuvel J.C. van den Neijssel O.M Cronenberg C.C.H Mattos M.J.T. de Beer D. de Boer J.P. de |
AuthorAffiliation | 1 Department of Microbiology, Biotechnology Center, University of Amsterdam, Nieuwe Achtergracht 127, 1018 WS Amsterdam, and Department of Chemical Engineering, Biotechnology Center, University of Amsterdam, 1018 WV Amsterdam, 2 The Netherlands |
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References | 16347213 - Appl Environ Microbiol. 1986 Nov;52(5):1139-46 5318988 - Lab Pract. 1965 Oct;14(10):1150-61 7027902 - Annu Rev Microbiol. 1981;35:299-324 1883604 - Biosens Bioelectron. 1991;6(3):255-62 |
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Snippet | Size distributions and glucose and pH profiles of aggregates of the D-(--)-lactic acid-producing organism Bacillus laevolacticus were measured. The organisms... Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit... Size distributions and glucose and pH profiles of aggregates of the d-(-)-lactic acid-producing organism Bacillus laevolacticus were measured. The organisms... Size distributions and glucose and pH profiles of aggregates of the d -(-)-lactic acid-producing organism Bacillus laevolacticus were measured. The organisms... Size distributions and glucose and pH profiles of aggregates of the D-lactic acid-producing organism Bacillus laevolacticus were measured. It was determined... Size distributions and glucose and pH profiles of aggregates of the D-(-)-lactic acid-producing organism Bacillus laevolacticus were measured. The organisms... |
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SubjectTerms | acide lactique acido lactico Acids bacillus Bacteria Biochemistry Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest biosintesis biosynthese biosynthesis Biotechnology disponibilidad de nutrientes disponibilite d' element nutritif flocculation floculacion floculation Fundamental and applied biological sciences. Psychology glucosa glucose lactic acid Mission oriented research nutrient availability Physiology and Biotechnology Physiology and metabolism |
Title | pH and glucose profiles in aggregates of Bacillus laevolacticus |
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