Silver nanoparticles induce tight junction disruption and astrocyte neurotoxicity in a rat blood-brain barrier primary triple coculture model

• Published in: International Journal of Nanomedicine Vol. 10; no. default; pp. 6105 - 6119
• Main Authors: Dan, Mo, Xu, Liming, Shao, Anliang, Cheng, Xiang, Zhang, Cuiping, Yokel, Robert, Takemura, Taro, Hanagata, Nobutaka, Niwa, Masami, Watanabe, Daisuke
• Format: Journal Article
• Language: English
• Published: New Zealand Informa UK Limited 01.01.2015; Dove Medical Press Limited; Dove Medical Press
• Subjects: Animals; Astrocytes; Astrocytes - drug effects; Astrocytes - metabolism; Astrocytes - pathology; Biological Transport; Blood-Brain Barrier; Blood-Brain Barrier - drug effects; Blood-Brain Barrier - metabolism; Brain; Brain - drug effects; Brain - metabolism; Brain - pathology; Cell Membrane Permeability; Cells, Cultured; Coculture Techniques; Drug delivery systems; Drugs; Endothelium, Vascular; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Gene Expression Profiling; Innovations; International Journal of Nanomedicine; Medicine (General); Metal Nanoparticles; Metal Nanoparticles - administration & dosage; Metal Nanoparticles - chemistry; Microscopy, Electron, Transmission; Models, Biological; Nanoparticles; Original Research; Physiological aspects; R5-920; Rats; Real-Time Polymerase Chain Reaction; Signal Transduction; Signal Transduction - drug effects; Silver; Silver - chemistry; Silver compounds; Tight Junctions; Tight Junctions - drug effects; Tight Junctions - metabolism; Vehicles; global gene expression analysis; Ag nanoparticles; antioxidant defense; astrocytes; BBB model

Silver nanoparticles (Ag-NPs) can enter the brain and induce neurotoxicity. However, the toxicity of Ag-NPs on the blood-brain barrier (BBB) and the underlying mechanism(s) of action on the BBB and the brain are not well understood. To investigate Ag-NP suspension (Ag-NPS)-induced toxicity, a triple...