Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications

• Published in: Journal of controlled release Vol. 266; pp. 17 - 26
• Main Authors: Liu, Chang, Zhang, Li, Liu, Hao, Cheng, Kun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 28.11.2017
• Subjects: adaptive immunity; Animals; Archaea; bacteria; bacteriophages; clinical trials; CRISPR-Cas Systems; CRISPR-Cas9; Delivery; eukaryotic cells; Gene Editing; Gene therapy; Gene Transfer Techniques; genes; genetic engineering; Genetic Therapy; Humans; immunotherapy; mutation; Nanoparticle; neoplasms; Non-viral delivery; patients; plasmids; RNA; T-lymphocytes; Viruses; Non-viral delivery; Gene-editing; Delivery; Gene therapy; Nanoparticle; CRISPR-Cas9

The CRISPR-Cas9 genome-editing system is a part of the adaptive immune system in archaea and bacteria to defend against invasive nucleic acids from phages and plasmids. The single guide RNA (sgRNA) of the system recognizes its target sequence in the genome, and the Cas9 nuclease of the system acts a...