Purification and characterization of a rotenone-insensitive NADH:Q6 oxidoreductase from mitochondria of Saccharomyces cerevisiae

• Published in: European journal of biochemistry Vol. 176; no. 2; pp. 377 - 384
• Main Authors: Vries, S. de, Grivell, L.A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.1988; Blackwell
• Subjects: analysis; Analytical, structural and metabolic biochemistry; binding sites; Biological and medical sciences; cell membranes; Centrifugation, Density Gradient; Chromatography, DEAE-Cellulose; Electrophoresis, Polyacrylamide Gel; Enzymes and enzyme inhibitors; enzymology; Flavonoids; Flavonoids - pharmacology; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Immunoassay; isolation & purification; Kinetics; metabolism; mitochondria; Mitochondria - enzymology; NAD (coenzyme); NAD(P)H Dehydrogenase (Quinone); NADH:Q6 oxidoreductase; oxidation; Oxidation-Reduction; Oxidoreductases; pharmacology; Quinone Reductases; Quinone Reductases - analysis; Quinone Reductases - isolation & purification; Quinone Reductases - metabolism; rotenone; Rotenone - pharmacology; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - ultrastructure; ultrastructure; Fungi; Characterization; Mitochondria; NADH dehydrogenase (quinone); Yeast; Purification; Ascomycetes; Saccharomyces cerevisiae; Thallophyta

A mitochondrial NADH: Q6 oxidoreductase has been isolated from cells of Saccharomyces cerevisiae by a simple method involving extraction of the enzyme from the mitochondrial membrane with Triton X‐100, followed by chromatography on DEAE‐cellulose and blue Sepharose CL‐6B. By this procedure a 2000‐fo...