Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing

• Published in: Plant biotechnology journal Vol. 15; no. 5; pp. 648 - 657
• Main Authors: Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., Weeks, Donald P.
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.05.2017; Society for Experimental Biology; Association of Applied Biology; John Wiley and Sons Inc
• Subjects: alleles; allohexaploid; Arabidopsis - genetics; Arabidopsis thaliana; Biofuels; Brassicaceae - genetics; Brassicaceae - metabolism; Camelina; Camelina sativa; Composition; CRISPR; CRISPR-Cas Systems; CRISPR/Cas9; Deoxyribonucleic acid; DNA; Enzymes; Experiments; FAD2 gene; Fatty acid composition; Fatty Acid Desaturases - genetics; Fatty acids; Fatty Acids - biosynthesis; Fatty Acids - genetics; Gene Editing; Genes; Genetic engineering; Genetic modification; Genomes; Germ-Line Mutation; homozygosity; Linoleic acid; Linolenic acid; Linolenic acids; Mutation; Nuclease; Oils & fats; oilseed crops; Oleic acid; Organic chemistry; Plant Leaves - genetics; Plants, Genetically Modified; Polymerase Chain Reaction; Polyploidy; Polyunsaturated fatty acids; RNA; RNA, Guide, CRISPR-Cas Systems; seed oils; Seeds; Seeds - genetics; Seeds - metabolism; somatic cells; Technology application; United States > US; Camelina sativa; CRISPR/Cas9; gene editing; fatty acid composition; allohexaploid; oleic acid

Summary The CRISPR/Cas9 nuclease system is a powerful and flexible tool for genome editing, and novel applications of this system are being developed rapidly. Here, we used CRISPR/Cas9 to target the FAD2 gene in Arabidopsis thaliana and in the closely related emerging oil seed plant, Camelina sativa...