Genetic relatedness and antimicrobial resistance determinants among clinical isolates of enterococci from Cuba

This study describes the genetic relationships and antimicrobial resistance determinants found among 99 clinical isolates of enterococci from 15 different hospitals in Cuba. Pulsed-field gel electrophoresis SmaI analysis demonstrated a high degree of genetic diversity. A limited number of multiresis...

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Published inClinical microbiology and infection Vol. 12; no. 8; pp. 793 - 797
Main Authors Quiñones-Pérez, D., Goñi, P., Rubio, M.C., Baquero, F., Gómez–Lus, R., Del Campo, R.
Format Journal Article
LanguageEnglish
Published Oxford, UK Elsevier Ltd 01.08.2006
Blackwell Publishing Ltd
Blackwell
Subjects
Aminoglycosides - metabolism
Aminoglycosides - pharmacology
Antimicrobial resistance
Biological and medical sciences
Cuba
Drug Resistance, Bacterial - genetics
Enterococcus - drug effects
Enterococcus - genetics
Enterococcus faecalis
Enterococcus faecium
Enterococcus spp
epidemiology
genetic relatedness
Humans
Infectious diseases
Medical sciences
resistance
Central America
Cuba
America
West Indies
Cuba
epidemiology
Antimicrobial resistance
Enterococcus spp
resistance
genetic relatedness
Enterococcus
Drug susceptibility test
Epidemiology
Infection
Resistance
Streptococcaceae
Bacteria
Micrococcales
Clinical isolate
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Abstract This study describes the genetic relationships and antimicrobial resistance determinants found among 99 clinical isolates of enterococci from 15 different hospitals in Cuba. Pulsed-field gel electrophoresis SmaI analysis demonstrated a high degree of genetic diversity. A limited number of multiresistant Enterococcus faecalis clones, showing resistance to three or more families of antimicrobial agents, were detected simultaneously in different institutions, suggesting inter-hospital circulation of selected clones, and/or selection of particular clones following their introduction into the hospital environment. Antimicrobial resistance determinants, including erm(B), aac(6′)–aph(2′), aph(3′), ant(6), vanB (E. faecalis) and vanA (Enterococcus faecium) were detected by PCR in various isolates.
AbstractList This study describes the genetic relationships and antimicrobial resistance determinants found among 99 clinical isolates of enterococci from 15 different hospitals in Cuba. Pulsed‐field gel electrophoresis SmaI analysis demonstrated a high degree of genetic diversity. A limited number of multiresistant Enterococcus faecalis clones, showing resistance to three or more families of antimicrobial agents, were detected simultaneously in different institutions, suggesting inter‐hospital circulation of selected clones, and/or selection of particular clones following their introduction into the hospital environment. Antimicrobial resistance determinants, including erm(B), aac(6′)–aph(2′), aph(3′), ant(6), vanB (E. faecalis) and vanA (Enterococcus faecium) were detected by PCR in various isolates.
Author Quiñones-Pérez, D.
Goñi, P.
Rubio, M.C.
Gómez–Lus, R.
Baquero, F.
Del Campo, R.
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  fullname: Del Campo, R.
  email: rosacampo@yahoo.com
  organization: Service of Microbiology, University Hospital Ramó y Cajal, Madrid, Spain
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CitedBy_id crossref_primary_10_1089_mdr_2012_0028
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crossref_primary_10_1007_s10096_013_1868_y
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Issue 8
Keywords Cuba
epidemiology
Antimicrobial resistance
Enterococcus spp
resistance
genetic relatedness
Enterococcus
Drug susceptibility test
Epidemiology
Infection
Resistance
Streptococcaceae
Bacteria
Micrococcales
Clinical isolate
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Snippet This study describes the genetic relationships and antimicrobial resistance determinants found among 99 clinical isolates of enterococci from 15 different...
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SubjectTerms Aminoglycosides - metabolism
Aminoglycosides - pharmacology
Antimicrobial resistance
Biological and medical sciences
Cuba
Drug Resistance, Bacterial - genetics
Enterococcus - drug effects
Enterococcus - genetics
Enterococcus faecalis
Enterococcus faecium
Enterococcus spp
epidemiology
genetic relatedness
Humans
Infectious diseases
Medical sciences
resistance
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Title Genetic relatedness and antimicrobial resistance determinants among clinical isolates of enterococci from Cuba
URI https://dx.doi.org/10.1111/j.1469-0691.2006.01421.x
https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1469-0691.2006.01421.x
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Volume 12
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