烟草种质资源TI203对烟草花叶病的抗性特点
【目的】系统了解烟草种质资源TI203抗烟草花叶病毒（Tobaccomosaicvirus，TMV）的特点，为烟草抗病育种的亲本选择提供更丰富信息。【方法】以烟草种质资源TI203为材料，采用TMV病毒接种、病情调查、病毒外壳蛋白累计量检测、TMV复制水平测定及日灼现象观察等方法，研究TI203对TMV的抗性特异性、TI203接种TMV普通株系（TMV-U1株系）后的症状、病毒复制水平等。【结果】TI203只对TMV表现无症状的抗病特点，接种马铃薯Y病毒（PVY）和黄瓜花叶病毒（CMV）则表现出典型症状。接种TMV-U1株系后14d，Westernblotting检测结果显示，TI203叶片病...
Saved in:
| Published in | 南方农业学报 Vol. 48; no. 11; pp. 2004 - 2009 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明,650021%云南省烟草公司 昭通市公司,云南 昭通,657000%浙江大学 农业与生物技术学院 生物技术研究所/农业部作物病虫分子生物学重点实验室,杭州,310058
2017
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-1191 |
| DOI | 10.3969/j.issn.2095-1191.2017.11.13 |
Cover
| Abstract | 【目的】系统了解烟草种质资源TI203抗烟草花叶病毒（Tobaccomosaicvirus，TMV）的特点，为烟草抗病育种的亲本选择提供更丰富信息。【方法】以烟草种质资源TI203为材料，采用TMV病毒接种、病情调查、病毒外壳蛋白累计量检测、TMV复制水平测定及日灼现象观察等方法，研究TI203对TMV的抗性特异性、TI203接种TMV普通株系（TMV-U1株系）后的症状、病毒复制水平等。【结果】TI203只对TMV表现无症状的抗病特点，接种马铃薯Y病毒（PVY）和黄瓜花叶病毒（CMV）则表现出典型症状。接种TMV-U1株系后14d，Westernblotting检测结果显示，TI203叶片病毒复制水平明显低于对照品种；用表达GFP的TMV侵染性克隆浸润接种发现，TI203对TMV的抗性发生在病毒复制水平。在高温和强日照条件下，TI203生长迅速的中上部叶片会发生日灼现象，且难以恢复。【结论】TI203只对TMV表现无症状，其对TMV的抗性发生在病毒复制水平。 |
|---|---|
| AbstractList | S286.3; [目的]系统了解烟草种质资源TI203抗烟草花叶病毒(Tobacco mosaic virus,TMV)的特点,为烟草抗病育种的亲本选择提供更丰富信息.[方法]以烟草种质资源TI203为材料,采用TMV病毒接种、病情调查、病毒外壳蛋白累计量检测、TMV复制水平测定及日灼现象观察等方法,研究TI203对TMV的抗性特异性、TI203接种TMV普通株系(TMV-U1株系)后的症状、病毒复制水平等.[结果]TI203只对TMV表现无症状的抗病特点,接种马铃薯Y病毒(PVY)和黄瓜花叶病毒(CMV)则表现出典型症状.接种TMV-U1株系后14 d,Western blotting检测结果显示,TI203叶片病毒复制水平明显低于对照品种;用表达GFP的TMV侵染性克隆浸润接种发现,TI203对TMV的抗性发生在病毒复制水平.在高温和强日照条件下,TI203生长迅速的中上部叶片会发生日灼现象,且难以恢复.[结论]TI203只对TMV表现无症状,其对TMV的抗性发生在病毒复制水平. 【目的】系统了解烟草种质资源TI203抗烟草花叶病毒（Tobaccomosaicvirus，TMV）的特点，为烟草抗病育种的亲本选择提供更丰富信息。【方法】以烟草种质资源TI203为材料，采用TMV病毒接种、病情调查、病毒外壳蛋白累计量检测、TMV复制水平测定及日灼现象观察等方法，研究TI203对TMV的抗性特异性、TI203接种TMV普通株系（TMV-U1株系）后的症状、病毒复制水平等。【结果】TI203只对TMV表现无症状的抗病特点，接种马铃薯Y病毒（PVY）和黄瓜花叶病毒（CMV）则表现出典型症状。接种TMV-U1株系后14d，Westernblotting检测结果显示，TI203叶片病毒复制水平明显低于对照品种；用表达GFP的TMV侵染性克隆浸润接种发现，TI203对TMV的抗性发生在病毒复制水平。在高温和强日照条件下，TI203生长迅速的中上部叶片会发生日灼现象，且难以恢复。【结论】TI203只对TMV表现无症状，其对TMV的抗性发生在病毒复制水平。 |
| Abstract_FL | [Objective]This research was conducted to understand the resistance characteristics of tobacco germplasm resource TI203 to Tobacco mosaic virus(TMV),in order to provide more information for parent selection in disease resis-tant breeding of tobacco.[Method]Tobacco germplasm resource TI203 was taken as experimental material. The resistance specificity of TI203 to TMV,and symptom and level of virus replication of TI203 after inoculated with TMV ordinary strain(TMV-U1 strain)were studied by TMV inoculation,disease investigation,virus coat protein accumulation detec-tion,TMV replication level determination and observation on sunburn phenomenon.[Result]The results showed that TI203 exhibited a symptomless reaction to TMV,but typical symptoms to Potato virus Y(PVY)and Cucumber mosic vi-rus(CMV)after inoculation. Western blotting detection results indicated that the level of virus replication in TI203 leaves was obviously lower than that of control variety 14 d after inoculated with TMV-U1 strain. Resistance of TI203 to TMV was observed at virus replication level by using TMV-GFP infectious clone infiltration inoculation. Under high tempera-ture and strong sunlight conditions,sunburn phenomena always occurred on fast-growing middle and upper leaves of TI203,and the leaves were difficult to recover.[Conclusion]TI203 is symptomless to TMV and its resistance to TMV occurs at virus replication level. |
| Author | 于海芹;饶思莲;赵丹阳;刘勇;李永平 |
| AuthorAffiliation | 云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明650021;云南省烟草公司昭通市公司,云南昭通657000;浙江大学农业与生物技术学院生物技术研究所/农业部作物病虫分子生物学重点实验室,杭州310058 |
| AuthorAffiliation_xml | – name: 云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明,650021%云南省烟草公司 昭通市公司,云南 昭通,657000%浙江大学 农业与生物技术学院 生物技术研究所/农业部作物病虫分子生物学重点实验室,杭州,310058 |
| Author_FL | RAO Si-lian LI Yong-ping ZHAO Dan-yang YU Hai-qin LIU Yong |
| Author_FL_xml | – sequence: 1 fullname: YU Hai-qin – sequence: 2 fullname: RAO Si-lian – sequence: 3 fullname: ZHAO Dan-yang – sequence: 4 fullname: LIU Yong – sequence: 5 fullname: LI Yong-ping |
| Author_xml | – sequence: 1 fullname: 于海芹;饶思莲;赵丹阳;刘勇;李永平 |
| BookMark | eNo9jz1LA0EQhreIYIz5FSms7tzZud27LSX4EQjYpA-b3buYUzeaQ0w60VgkEGIXsLGMnQgqxP-Tu_sZnkSs5mXehxmeHVKyfRsSUgPqohRyP3Z7SWJdRiV3ACQUCXwXwAUskfL_eptUkySmlDJOfYGsTILs4SWfTbLlLP94zT_H6fdTq8Eort9Wmyafvq_nX9niMXsep9NFerfMJqvsfrVLtiJ1kYTVv1khraPDVv3EaZ4eN-oHTUcLik4HtDKB4kKACTyjFKIMFUVuOuhpKlUn8kLm8VBwozSXTGrto2FceIZ5kcAK2ducvVU2Urbbjvs3A1s8bHeHdnQ-_PUEoIAFWduQ-qxvu9e9gr0a9C7VYNT2C2MUAXKJP24QaPk |
| ClassificationCodes | S286.3 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W95 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3969/j.issn.2095-1191.2017.11.13 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-农业科学 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Resistance characteristics of tobacco germplasm resource TI203 to Tobacco mosaic virus |
| DocumentTitle_FL | Resistance characteristics of tobacco germplasm resource TI203 to Tobacco mosaic virus |
| EndPage | 2009 |
| ExternalDocumentID | gxnykx201711013 7000368359 |
| GrantInformation_xml | – fundername: 中国烟草总公司科技项目; 中国烟草总公司云南省公司科技项目 funderid: (110201401001); (2014YN02) |
| GroupedDBID | -04 2RA 5XA 5XE 92L ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CQIGP U1G U5N W95 ~WA 2B. 4A8 92I 93N PSX TCJ |
| ID | FETCH-LOGICAL-c603-b1cad8a5661d84daa339ea035db34c09abf4e245e65dac5929cc73d2564d24f63 |
| ISSN | 2095-1191 |
| IngestDate | Thu May 29 04:06:49 EDT 2025 Wed Feb 14 09:56:29 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 11 |
| Keywords | TI203 tobacco germplasm 烟草花叶病毒(TMV) disease resistance 抗病性 Tobacco mosaic virus(TMV) 烟草种质 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c603-b1cad8a5661d84daa339ea035db34c09abf4e245e65dac5929cc73d2564d24f63 |
| Notes | 45-1381/S Objective】This research was conducted to understand the resistance characteristics of tobacco germplasm resource TI203to Tobacco mosaic virus(TMV),in order to provide more information for parent selection in disease resistant breeding of tobacco.【Method】Tobacco germplasm resource TI203was taken as experimental material.The resistance specificity of TI203to TMV,and symptom and level of virus replication of TI203after inoculated with TMV ordinary strain(TMV-U1strain)were studied by TMV inoculation,disease investigation,virus coat protein accumulation detection,TMV replication level determination and observation on sunburn phenomenon.【Result】The results showed that TI203exhibited a symptomless reaction to TMV,but typical symptoms to Potato virus Y(PVY)and Cucumber mosic virus(CMV)after inoculation.Western blotting detection results indicated that the level of virus replication in TI203leaves was obviously lower than that of control variety14d after inoculated with TMV-U1strain.Resistance of TI203to TMV |
| PageCount | 6 |
| ParticipantIDs | wanfang_journals_gxnykx201711013 chongqing_primary_7000368359 |
| PublicationCentury | 2000 |
| PublicationDate | 2017 |
| PublicationDateYYYYMMDD | 2017-01-01 |
| PublicationDate_xml | – year: 2017 text: 2017 |
| PublicationDecade | 2010 |
| PublicationTitle | 南方农业学报 |
| PublicationTitleAlternate | Journal of Southern Agriculture |
| PublicationTitle_FL | Journal of Southern Agriculture |
| PublicationYear | 2017 |
| Publisher | 云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明,650021%云南省烟草公司 昭通市公司,云南 昭通,657000%浙江大学 农业与生物技术学院 生物技术研究所/农业部作物病虫分子生物学重点实验室,杭州,310058 |
| Publisher_xml | – name: 云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明,650021%云南省烟草公司 昭通市公司,云南 昭通,657000%浙江大学 农业与生物技术学院 生物技术研究所/农业部作物病虫分子生物学重点实验室,杭州,310058 |
| SSID | ssj0002507632 ssib007891390 ssib051370557 ssib011608256 ssib018830053 ssib010683288 |
| Score | 2.0608842 |
| Snippet | ... S286.3; [目的]系统了解烟草种质资源TI203抗烟草花叶病毒(Tobacco mosaic... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 2004 |
| SubjectTerms | 烟草种质;TI203;烟草花叶病毒(TMV);抗病性 |
| Title | 烟草种质资源TI203对烟草花叶病的抗性特点 |
| URI | http://lib.cqvip.com/qk/90177A/201711/7000368359.html https://d.wanfangdata.com.cn/periodical/gxnykx201711013 |
| Volume | 48 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFA-lingRRcVqlYLOqaTmYyaZOU52s1RBT1uopyWbZFsQtn60UHsSrYcWip4sePFYbyKoUP-fdvfP8L2X2WzsBm2Fsry-fXnzex_ZeTOZmVjWva5Q4PsktXMvTGweJtJOApHbWc8VMlDQJ9CE26PHweISf7gslqfOf6ysWtpY7y6kW7X7Sv4nqsCDuOIu2TNEtlQKDKAhvvAJEYbPU8WYxSGTPlMtFksmm0wq5OgQaeBEnGlJhGCSszhgkWbKaT_wHJ_FgukWi1SdDiA0i1yUkSAToIwCMUGENspABphIONgm6lEjhR4Q1bqXVDWNvApIDDhAN1jMWSRRLUJqMh0Y5VqMsoFEoNmYbBAsCkcY1VhEwQWElQAp8gBcEXljEfIE_BUtIgbFFBD-WARASeQhETHTS5tZkWL7J2WwQaTcimEhGiPdOodOmkphKi43NgMHYHMiFBkC4MAoPLq2PkjaZfJPnZPJAApVy8QG1BaeBf_EhEcXwhAtr5I5CuwmwgSSQMZMxeRkTY0SR3PCJpiKWNQ0zCJdJj0A_0baCIOlMYVRU0pBloKzazxApmnSHLVQp9cg5BL9aQhczgsDAKgimSfOHhfA3Bglupw3SnXhBcdkZUQ-mpAmNCfbq1OBAEMH34cuTAYrCgXcYor8B47EO8jkwnxdwhCBd8BpYlomlUmh-dNdNhmAAIVpeXAdJoUOxZ8YjuHHr0rl5Q-GJASRubMwbAROOXWQKrjPkIJFTjRHiRKimNfAZ4qiUnR5MMyz8aDJalXGZbX3cas1llO8sNzU6_h4t64W9FWgqBbENhbKNnA1bwiF4kKxBf7EYfsrm_1XTzdRBIZF-DL0c14YurhUvPlkqaxTQlzbMV5d4DoB1EXjYx9dN8CJt3KY50qJLwUp6yDh-niGWVg-C4BhKJR5-MC7hHnBumtsuP8XC_BAoNW1_spzGObQrtN-L-mvVAZI7cvWJTOzMaeLbuqKNbW1etWSg7efh3s7g4O94fcvwx_bx78-UPdz9PWw-Ga4--3o_c_B_rvBp-3j3f3j1weDncPBm8NrVrsVtxuLtnlZj50Gjm933TTJZCJguJdJniWJ76s8cXyRdX2eOirp9njucZEHIktSKBBUmoZ-Bl7imcd7gX_dmu6v9fMb1hwMaFUqeS_Jeil3s0D1wLth6gg8nTIX6Yw1WxrdeVacydQJ6WQ16Qs1Y80ZP3RMV_2ycyKsN_8tcsu6iHQx2T5rTa-_2Mhvw_BzvXuHcuE36mc8Ug |
| linkProvider | CAB International |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E7%83%9F%E8%8D%89%E7%A7%8D%E8%B4%A8%E8%B5%84%E6%BA%90TI203%E5%AF%B9%E7%83%9F%E8%8D%89%E8%8A%B1%E5%8F%B6%E7%97%85%E7%9A%84%E6%8A%97%E6%80%A7%E7%89%B9%E7%82%B9&rft.jtitle=%E5%8D%97%E6%96%B9%E5%86%9C%E4%B8%9A%E5%AD%A6%E6%8A%A5&rft.au=%E4%BA%8E%E6%B5%B7%E8%8A%B9&rft.au=%E9%A5%B6%E6%80%9D%E8%8E%B2&rft.au=%E8%B5%B5%E4%B8%B9%E9%98%B3&rft.au=%E5%88%98%E5%8B%87&rft.date=2017&rft.pub=%E4%BA%91%E5%8D%97%E7%9C%81%E7%83%9F%E8%8D%89%E5%86%9C%E4%B8%9A%E7%A7%91%E5%AD%A6%E7%A0%94%E7%A9%B6%E9%99%A2%2F%E7%83%9F%E8%8D%89%E8%A1%8C%E4%B8%9A%E7%83%9F%E8%8D%89%E7%94%9F%E7%89%A9%E6%8A%80%E6%9C%AF%E8%82%B2%E7%A7%8D%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2F%E5%9B%BD%E5%AE%B6%E7%83%9F%E8%8D%89%E5%9F%BA%E5%9B%A0%E5%B7%A5%E7%A8%8B%E7%A0%94%E7%A9%B6%E4%B8%AD%E5%BF%83%2C%E6%98%86%E6%98%8E%2C650021%25%E4%BA%91%E5%8D%97%E7%9C%81%E7%83%9F%E8%8D%89%E5%85%AC%E5%8F%B8+%E6%98%AD%E9%80%9A%E5%B8%82%E5%85%AC%E5%8F%B8%2C%E4%BA%91%E5%8D%97+%E6%98%AD%E9%80%9A%2C657000%25%E6%B5%99%E6%B1%9F%E5%A4%A7%E5%AD%A6+%E5%86%9C%E4%B8%9A%E4%B8%8E%E7%94%9F%E7%89%A9%E6%8A%80%E6%9C%AF%E5%AD%A6%E9%99%A2+%E7%94%9F%E7%89%A9%E6%8A%80%E6%9C%AF%E7%A0%94%E7%A9%B6%E6%89%80%2F%E5%86%9C%E4%B8%9A%E9%83%A8%E4%BD%9C%E7%89%A9%E7%97%85%E8%99%AB%E5%88%86%E5%AD%90%E7%94%9F%E7%89%A9%E5%AD%A6%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2C%E6%9D%AD%E5%B7%9E%2C310058&rft.issn=2095-1191&rft.volume=48&rft.issue=11&rft.spage=2004&rft.epage=2009&rft_id=info:doi/10.3969%2Fj.issn.2095-1191.2017.11.13&rft.externalDocID=gxnykx201711013 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90177A%2F90177A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fgxnykx%2Fgxnykx.jpg |