Identification and cloning of the gene involved in the final step of chlortetracycline biosynthesis in Streptomyces aureofaciens

• Published in: Bioscience, biotechnology, and biochemistry Vol. 68; no. 6; pp. 1345 - 1352
• Main Authors: Nakano, T. (Kyowa Hakko Kogyo Co. Ltd., Machida, Tokyo (Japan). Tokyo Research Lab.), Miyake, K, Endo, H, Dairi, T, Mizukami, T, Katsumata, R
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.06.2004; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: amino acid sequences; antibacterial properties; ANTIBIOTIC PROPERTIES; Base Sequence; Biological and medical sciences; BIOSYNTHESIS; biotechnology; chlortetracycline; Chlortetracycline - biosynthesis; Cloning, Molecular; coenzyme F; DNA; Fundamental and applied biological sciences. Psychology; GENES; IDENTIFICATION; MOLECULAR CLONING; Molecular Sequence Data; molecular weight; Multigene Family; Mutation; Mycobacterium bovis; NUCLEOTIDE SEQUENCE; Open Reading Frames; OXIDOREDUCTASES; Oxidoreductases - genetics; sequence analysis; Southern blotting; STREPTOMYCES; Streptomyces aureofaciens; Streptomyces aureofaciens - genetics; Streptomyces aureofaciens - metabolism; tchA; tetracycline; tetracycline dehydrogenase; TETRACYCLINES; Streptomycetaceae; Enzyme; Biosynthesis; Identification; coenzyme F420; Dehydrogenase; chlortetracycline biosynthesis; Coenzyme; Tetracycline; Actinomycetales; tetracycline dehydrogenase; Bacteria; Chlortetracycline; Actinomycetes; Oxidoreductases; Molecular cloning; tchA; Streptomyces aureofaciens

For chlortetracycline biosynthesis in Streptomyces aureofaciens, the final reduction step is essential to give an antibiotic activity to its intermediate, which is catalyzed by tetracycline dehydrogenase with 7,8-dedimethyl-8-hydroxy-5-deazariboflavin (FO) as a cofactor. We identified and cloned the...